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Plasmid#41665DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; C-terminal hyd…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-mCherry-FLARE-EKAR-EV
Plasmid#123341PurposeRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.DepositorInsertmCherry-mCherry-FLARE-EKAR-EV
UseTags6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Cp110-3'UTR
Plasmid#64083PurposeLuciferase assayDepositorInsertCcp110 (centriolar coiled coil protein 110) (Ccp110 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR-27a-3p
Plasmid#82438PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3pDepositorInserthsa-miR-27a-3p (MIR27A Human)
UseTagsExpressionMutationThree copies hsa-miR-27a-3p Complementary region …PromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-EEA1
Plasmid#109560PurposeMultisite gateway vector for 5' tagging with early endosome associated 1DepositorInsertearly endosome associated 1 (eea1 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP AH2 tensin
Plasmid#50514PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertTensin (TNS1 Chicken)
UseTagsGFPExpressionMammalianMutationAA659--761, including actin homology 2 domain of …PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold
Plasmid#121812PurposepMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Gp88 in situ probe
Plasmid#45628DepositorInsertGpr88 in situ probe (Gpr88 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 2742-3255 of AB042408.1 or …PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
JE301: pMVP (L5-L4) Tir1-P2A-AID
Plasmid#121722PurposepMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.DepositorInsertosTir1-P2A-AID (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTags9x myc (C-term on osTIR1)ExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Epha3 in situ probe
Plasmid#45627DepositorInsertEpha3 in situ probe (Epha3 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 3179-3773 of NM_010140PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core
Plasmid#121839PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75233PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
5mARF17 pGreenII0229
Plasmid#12071DepositorInsert5mARF17 (ARF17 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionPlantMutationmiR160-resistant version of ARF17 has five silent…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-577-CFP-578CT
Plasmid#41682DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; CFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-577-YFP-578CT
Plasmid#41681DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; YFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1CFP*
Plasmid#41666DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; C-terminal hyd…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-2
Plasmid#109009PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL2-sgRNA #2 (ATL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
5mARF17 pGreenII0129
Plasmid#12081DepositorInsert5mARF17 (ARF17 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionPlantMutationmiR160-resistant version of ARF17 has five silent…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV
Plasmid#216162PurposeExpresses Cre recombinase in cells lacking TDP-43 functionDepositorInsertCre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2 Cryptic PE-Max
Plasmid#216163PurposeExpression of prime editing vector PEMax (Plasmid #174820) but only in cells with TDP-43 loss of functionDepositorInsertPEMax for prime editing with internal cryptic exon
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only