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Plasmid#128095PurposeHypoxia response element real-time luciferase reporterDepositorInsertHypoxia response element (x3) from PGK1 promoter (PGK1 Human)
UseLuciferaseTagsdrives expression of destabilized luciferase (2CP…ExpressionMammalianMutationPromoterinserted PGK1 hypoxia response elements (HRE) (x3)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA-CasRx_SD1-pSV40-TagBFP
Plasmid#221004PurposeTransiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.DepositorInsertCasRx 30nt processed direct repeat with SD1 mutation
UseCRISPRTagsExpressionMammalianMutationDR1 A>TPromoterU6Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDae079 (eMutaT7[transition])
Plasmid#187622PurposeExpresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertsTadA-8e-T7RNAP
PmCDA1-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)
Plasmid#179116PurposeCargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site for in-frame fusion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG (AAV8)
Viral Prep#105537-AAV8PurposeReady-to-use AAV8 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsNoneAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLT3-ITD-MSCV-IRES-GFP
Plasmid#184779PurposeExpresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cellsDepositorInsertFLT3 (FLT3 Human)
UseRetroviralTagsExpressionMammalianMutationActivating internal tandem duplication (ITD) adde…PromoterMSCVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-ER GFP
Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-24xGCN4_v4-kif18b-24xPP7
Plasmid#74928PurposeTranslation reporterDepositorInsert24xGCN4_v4-kif18b-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-HRas
Plasmid#18662DepositorInsertH-Ras (HRAS Human)
UseTagsmonomeric EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190034Purposevector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13d
UseTagsExpressionMammalianMutationA134V,A140V,A141V,A143VPromoterCBh, SV40, hU6Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-WT-STAT1
Plasmid#71454PurposeLentiviral expression of WT-STAT1DepositorInsertSTAT1 (STAT1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEJS2422_Nme2.Smu-ABE8e-i1
Plasmid#201520PurposeMammalian expression of domain inlaid Nme2.Smu-ABE8e-i1DepositorInsertNme2.Smu-ABE8e-i1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_StrepII_TEV_PP2Ac
Plasmid#217477PurposeExpresses PP2Ac with a TEV cleavable N-terminal StrepII tagDepositorInsertPP2Ac
UseTagsStrepII, TEVExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage ubc nls ha 2xmcp HALO
Plasmid#64540PurposeUsed to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.DepositorInsertMCP
UseLentiviralTagsHA, HALO, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSGV Mouse Acceptor
Plasmid#128176PurposeA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en31FnCas9
Plasmid#201949PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en31FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationG1243T, E1369R, E1603H on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8s
Plasmid#169247PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmStrawberry-Atg4BC74A
Plasmid#21076DepositorInsertAtg4B(C74A) (Atg4b Mouse)
UseTagsmStrawberryExpressionMammalianMutation220-222 TGC(Cys) is changed to GCA (Ala)PromoterAvailable sinceJune 8, 2009AvailabilityAcademic Institutions and Nonprofits only