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Plasmid#179967PurposeMammalian expression vector for SARS-CoV-1 M, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 M (M Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mKalama1
Plasmid#102639PurposeIn vitro expression of mKalama1 from the T7 promoterDepositorInsertmKalama1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV2 M C-V5-tag
Plasmid#179978PurposeMammalian expression vector for SARS-CoV-2 M, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-2 M (M Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pEF-GFP(rc)Bxb1
Plasmid#127511PurposePlasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV2 ORF7a C-V5-tag
Plasmid#179981PurposeMammalian expression vector for SARS-CoV-2 ORF7a, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-2 ORF7a (ORF7a Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 NSP15 C-V5-tag
Plasmid#179964PurposeMammalian expression vector for SARS-CoV-1 Nsp15, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 Nsp15 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CyPet
Plasmid#102636PurposeIn vitro expression of CyPet from the T7 promoterDepositorInsertCyPet
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)phiC31
Plasmid#127527PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)Bxb1
Plasmid#127528PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CASANOVA
Plasmid#113036PurposeAAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 NSP1 C-V5-tag
Plasmid#179982PurposeMammalian expression vector for RaTG13 Nsp1, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 Nsp1
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV2 NSP1 C-V5-tag
Plasmid#179972PurposeMammalian expression vector for SARS-CoV-2 Nsp1, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-2 Nsp1 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mOrange
Plasmid#102632PurposeIn vitro expression of mOrange from the T7 promoterDepositorInsertmOrange
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 NSP1 C-V5-tag
Plasmid#179961PurposeMammalian expression vector for SARS-CoV-1 Nsp1, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 Nsp1 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 NSP15 C-V5-tag
Plasmid#179984PurposeMammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 Nsp15
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FRA1
Plasmid#188665PurposeExpresses murine FRA1 (Fosl1)DepositorInsertFosl1 (Fosl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterhPGKAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_MECP2_MBD-R106W
Plasmid#179408PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)DepositorInsertMECP2_MBD(R106W) (Mecp2 Mouse)
UseTagsAviTag and EGFPExpressionMammalianMutationMBD R106WPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-5xE
Plasmid#91993PurposeExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)PromoterAvailable sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-6HIS-TEV-mEGFP,IRES-NGFR
Plasmid#164521PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only