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Plasmid#190013PurposeExpresses codon-optimized full length SARS-CoV-2 BA.4.6 SpikeDepositorInsertSARS-CoV-2 BA.4.6 Spike (S )
UseTagsExpressionMammalianMutationContains the following mutations: T19I, LPPA24S, …Promoterchicken β-actin promoter, CMV enhancerAvailable sinceSept. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVI
Plasmid#198466PurposeExpresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-XBB.1.5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationG339H, R346T, L368I, S371F, S373P, S375F, T376A, …PromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.2
Plasmid#184829PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA.2 (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-XBB-AVI
Plasmid#196622PurposeExpresses SARS-CoV-2 S2P protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-XBB (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…PromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/EG.5.1
Plasmid#218668PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG.5.1 variantDepositorInsertpαH-S-GSAS/EG.5.1 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterCMVAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR-OMICRON.BA.5
Plasmid#213071PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intactDepositorInsertpαH-S-RRAR-OMICRON.BA.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685, furin site inta…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/XBB.1.5
Plasmid#212991Purpose"Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 variantDepositorInsertpαH-S-GSAS/XBB.1.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutation(T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q,Q183E…PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/XBB.1.16
Plasmid#212992PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16DepositorInsertpαH-S-GSAS/XBB.1.16 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutation(T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q, E180…PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR/XBB.1.16
Plasmid#213037PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intactDepositorInserts -RRAR.XBB.1.16 (S )
UseTagsHRV 3C cleavage site (before tags) 8X His tag 2X…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 furin site RRAR …PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/EG.5
Plasmid#212990PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG5 variantDepositorInsertpαH-S-GSAS/EG.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-BQ.1.1-AVI
Plasmid#196621PurposeExpresses SARS-CoV-2 S2P protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BQ.1.1 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24-, P25-, P26-, A27S, H69-, V70-, G142D, …PromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-BJ.1-AVI
Plasmid#196620PurposeExpresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BJ.1 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…PromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.2 Spike
Plasmid#185604PurposeExpresses codon-optimized full length SARS-CoV-2 BA.2 SpikeDepositorInsertSARS-CoV-2 BA.2 Spike (S )
UseTagsExpressionMammalianMutationT19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJuly 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS SARS-CoV-2 BA.2.75 Spike
Plasmid#190012PurposeExpresses codon-optimized full length SARS-CoV-2 BA.2.75 SpikeDepositorInsertSARS-CoV-2 BA.2.75 Spike (S )
UseTagsExpressionMammalianMutationContains the BA.2 mutations (T19I, LPPA24S, G142D…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceSept. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI
Plasmid#198465PurposeExpresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-XBB.1.5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…PromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR/XBB.1.5
Plasmid#212993PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intactDepositorInsertSpike S-RRAR/XBB.1.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); furin site intake; (T19I…PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
The Mammalian Toolkit
Plasmid Kit#1000000180PurposeA ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.DepositorAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Winslow Lab Retroviral Barcoding Library
Plasmid Kit#1000000097PurposeBarcoding library of retroviral plasmids with unique 6 nucleotide barcodes, for in vivo phenotypic screening of small-molecule libraries.DepositorAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CCSB-Broad Human Kinase ORF Collection
Plasmid Kit#1000000014Purpose556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Musunuru-Cowan TALEN Kit
Plasmid Kit#1000000034PurposeUsed to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats. Contains pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.DepositorAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only