Addgene

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pAAV.CamKII(1.3).eYFP.WPRE.hGH

Plasmid

#105622

Purpose

AAV expression of EYFP from CaMKII(1.3) promoter

Depositor

Karl Deisseroth

Has Service

AAV1

Insert

EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKII(1.3)

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Tnf

Plasmid

#207127

Purpose

Luciferase vector containing 3'UTR for mouse Tnf

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Tumor necrosis factor (Tnf) 3'UTR (Tnf Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ALIX(1-702)-mNeonGreen

Plasmid

#191193

Purpose

Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (1-702) (PDCD6IP Synthetic, Human)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Deletion of ALIX C-terminal PRD.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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ALIX(1-702)-mNeonGreen

Plasmid

#191193

Purpose

Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (1-702) (PDCD6IP Synthetic, Human)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Deletion of ALIX C-terminal PRD.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTORGm43GW

Plasmid

#112902

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GW

Plasmid

#112902

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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198_pAAV-ProD1-CatCh-GFP-WPRE

Plasmid

#125977

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD1

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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198_pAAV-ProD1-CatCh-GFP-WPRE

Plasmid

#125977

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD1

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTurquoise2

Plasmid

#118453

Purpose

Overexpression of yeast codon-optimized mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTurquoise2

Plasmid

#118453

Purpose

Overexpression of yeast codon-optimized mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFF530

Plasmid

#61447

Purpose

IPTG-inducible msd_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFF530

Plasmid

#61447

Purpose

IPTG-inducible msd_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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sh-SRF

Plasmid

#100797

Purpose

expression of shRNA targeting SRF

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

Insert

rat SRF

Use

RNAi

Tags

Expression

Mutation

Promoter

H1

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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sh-SRF

Plasmid

#100797

Purpose

expression of shRNA targeting SRF

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

Insert

rat SRF

Use

RNAi

Tags

Expression

Mutation

Promoter

H1

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM-sgMUC1_dual-MTS-3x-Set I

Plasmid

#162763

Purpose

Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-sgMUC1_dual-MTS-3x-Set I

Plasmid

#162763

Purpose

Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

Plasmid

#179586

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NeonGreen

Expression

Mutation

Promoter

TRE

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

Plasmid

#179586

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NeonGreen

Expression

Mutation

Promoter

TRE

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp29-C1

Plasmid

#134545

Purpose

Expresses YFP-Rpp29 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp29 (POP4 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp29-C1

Plasmid

#134545

Purpose

Expresses YFP-Rpp29 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp29 (POP4 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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