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Plasmid#188092PurposeTo make monomeric ATG9 C-terminal tail for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (692-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (723-839)
Plasmid#188088PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (723-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9 (801-839)
Plasmid#188093PurposeTo make monomeric ATG9 C-terminal tail for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (801-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (828-839)
Plasmid#188090PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (828-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-ATG13 (2-197)
Plasmid#188075PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorInsertATG13 (ATG13 Human)
UseTagsstrep-strep-Flag tagExpressionMammalianMutationATG13 (2-197)PromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTBL405 CHYRON1 integration construct
Plasmid#126442PurposeTo integrate the CHYRON1 locus at HEK293site3.DepositorInsertspU6-CHYRON1 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6Available sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoBa
Plasmid#121897PurposePlasmid for making zebrafish apoBa in situ probeDepositorInsertApoBa (apoba Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-N3FLAG-TAF12(50-130)
Plasmid#105595Purposeretrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistanceDepositorInsertTAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationtruncation fragments containing aa (50-130), and …PromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9-KRAB-MeCP2
Plasmid#110824PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pinducer 20 DN-KASHΔPPPL
Plasmid#129280PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPLDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryExpressionMutationthe last 4 amino acids (PPPL) of the KASH domain …PromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl-XL-s2193
Plasmid#166749PurposeExpress mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.DepositorInsertBcl-XL (BCL2L1 Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterHuman ferritinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424TagsExpressionMutationThe conserved GDD in the NS5 polymerase gene muta…PromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIN-PAmCherry-mSNCA-NE
Plasmid#102364PurposeLentiviral overexpression of PA-mCherry synuclein alpha fusionDepositorInsertsUseLentiviralTagsNEExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
BCR/ABL P190 transgenic construct
Plasmid#38185DepositorUseConstruct for making transgenic miceTagsExpressionMutationPromotertruncated mouse MT promoterAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcdna6.2- myc ULK1 k46I
Plasmid#27630DepositorInsertmouse myc ULK1 K46I (Myc Mouse)
UseTagsmycExpressionMammalianMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pegRNA-GFP>BFP_Y66H-NGG
Plasmid#185480PurposepegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.DepositorInsertGFP>BFP_Y66H-pegRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl-W-FUGW
Plasmid#166751PurposeExpress mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.DepositorInsertBcl-W (Bcl2l2 Mouse)
UseLentiviralTagsmCerulean3ExpressionMutationPromoterhuman ubiquitin CAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-GFP
Plasmid#130977PurposeExpression of KIF1C-GFP in mammalian cells.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTagsGFPExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis
Plasmid#157534PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3E (SEMA3E Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3F-Fc(DAPA)-AviTag-6xHis
Plasmid#156977PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertSEMA3F (SEMA3F Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only