Addgene

We narrowed to 3,692 results for: c1s

Showing: 3221 - 3240 of 3692 results

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GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-2xmCherry-EF(C)

Plasmid

#61664

Purpose

Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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Clover-Tubulin-C-18

Plasmid

#56374

Purpose

Localization: Microtubules, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Use

Tags

Clover

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SSpB-mCherry-p63DH

Plasmid

#176112

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha1-850

Plasmid

#62522

Purpose

C-terminal aa851-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 851-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 851-1374

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
Plxnc1-Fc-His

Plasmid

#72131

Purpose

Expresses the extracellular region of the PlexinC1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnc1 (Plxnc1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-GFP11

Plasmid

#61670

Purpose

Non-fluorescent, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-GFP11 (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hPMLIII

Plasmid

#103808

Purpose

BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLASP2 512-650

Plasmid

#24409

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant with only MT plus end trac…

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-CFP-FRBx5

Plasmid

#103780

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-2xmCitrine-GFP11

Plasmid

#61667

Purpose

Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinK388R

Plasmid

#134463

Purpose

M1 SpastinK388R expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Lys to Arg at amino acid 388

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-CD36-C-10

Plasmid

#55405

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

CD36 (CD36 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pmCherry-3xNLS-OptoJNKi

Plasmid

#89740

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB3-C-20

Plasmid

#55417

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-PH-SWAP70(R223E, R224E)

Plasmid

#84583

Purpose

Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Pleckstrin-homology domain, residues 209-306, mut…

Promoter

CMV

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
GFP-FKBP-TPD52

Plasmid

#172455

Purpose

Expression of Tumor Protein D52 (TPD52) with N-terminal GFP-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Use

Tags

GFP-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pONL-C1_Rab11a

Plasmid

#65707

Purpose

Expresses ONL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-NES-OptoJNKi

Plasmid

#89739

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-TetR

Plasmid

#103798

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-TetR (CIB1, tetR E. coli, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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