Addgene

We narrowed to 11,285 results for: pcDNA

Showing: 3201 - 3220 of 11285 results

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pcDNA3.1-R1ACR_741-EYFP

Plasmid

#103961

Purpose

Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_741

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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hJAM-A deltaCT pcDNA3.1

Plasmid

#70074

Purpose

Expresses human junctional adhesion molecule-A amino acids 1-260 in mammalian cells

Depositor

Terence Dermody

Article

Danthi et al J Virol. 2006 Feb;80(3):1261-70.

Insert

junctional adhesion molecule-A (F11R Human)

Use

Tags

Expression

Mammalian

Mutation

deleted C-terminal cytoplasmic tail (amino acids …

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZifCY3 (Ala4)

Plasmid

#36318

Depositor

Amy Palmer

Article

Dittmer et al J Biol Chem. 2009 Jun 12;284(24):16289-9

Insert

ZifCY3 genetically encoded Zn(II) sensor

Use

Tags

Expression

Mammalian

Mutation

see comment

Promoter

CMV

Available since

May 24, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1hisA cdc25c C330S

Plasmid

#10966

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S (CDC25C Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

C330S. Cysteine to Serine substitution at aa330.

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
Flag-NHR2+R pcDNA3.1

Plasmid

#17317

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
EphA2 1-427 pcDNA3.1

Plasmid

#102737

Purpose

Expression of N terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (1-427aa) (Epha2 Mouse)

Use

Tags

6xHis and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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AU1-TTC30A2-pcDNA3

Plasmid

#47324

Purpose

Expresses AU1 epitope-tagged TTC30A2 in mammalian cells

Depositor

Richard Maurer

Article

Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

Insert

TTC30A2 (Ttc30a2 Mouse)

Use

Tags

AU1 epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43-YA/VD-Myc

Plasmid

#60701

Purpose

mammalian expression of Myc tagged rat Cx43 YA/VD mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA-H1-sgRNA-hTZAP

Plasmid

#87186

Purpose

guideRNA targeting exon1 of human TZAP

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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Human NCKX3 (pcDNA3.1+)

Plasmid

#75205

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (SLC24A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5 713

Plasmid

#65246

Purpose

Expression of HA tagged ERK5 AA 2-713 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 713 (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 714-817

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal L150E

Plasmid

#47346

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

L150E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
NHR1+2-Flag pcDNA3.1

Plasmid

#17316

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pY124 (pcDNA3.1-huBsCpf1)

Plasmid

#92300

Purpose

Expression of humanized BsCpf1

Depositor

Feng Zhang

Insert

huBsCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 NDUFS6(5’UTR)-FF

Plasmid

#85488

Purpose

Firefly luciferase under the control of NDUFS6 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of NDUFS6 (NDUFS6 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 UQCC2(5’UTR)-FF

Plasmid

#85487

Purpose

Firefly luciferase under the control of UQCC2 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of UQCC2 (C6orf125 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRFP-Syx2

Plasmid

#35704

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx2 (Plekhg5 Mouse)

Use

Tags

mRFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-PPARgamma2 2KR

Plasmid

#78772

Purpose

To overexpress PPARgamma2 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2 (Ppargc1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

K184A, K185A

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/GS-DJ1-A104T

Plasmid

#29411

Depositor

Mark Cookson

Insert

DJ1 (PARK7 Human)

Use

Tags

Expression

Mammalian

Mutation

Ala104Thr

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only