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AID-mTagBFP2-loxP_myo2_neoR-loxP
Plasmid#194055PurposeDual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genomeDepositorInsertIAA17 (AXR3 Mustard Weed)
UseTagscodon optimized mTagBFP2ExpressionWormMutationPromoterNoneAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
UseTagsStrep-Tag II SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (TB40/E)
Plasmid#136446PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)
Plasmid#47550PurposeCas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion alleleDepositorInsertsCas9
ttTi5605 sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/MSCV-EGFP
Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRc20_992
Plasmid#49739DepositorInsertAS20_992
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas5
Plasmid#178878PurposeExpression of human codon-optimized Nlacas5 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas5c-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas8
Plasmid#178880PurposeExpression of human codon-optimized NlaCas8 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas8c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
NylCA_pET28a
Plasmid#215418PurposeExpression construct for NylCA gene, codon optimized for expression in E. coliDepositorInsertNylCA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2_pET28a
Plasmid#215420PurposeExpression construct for NylCp2 gene, codon optimized for expression in E. coliDepositorInsertNylCp2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832APromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A3
Plasmid#132160PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A3 (SLC6A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcI-ts,ind+-(NSP8)
Plasmid#160656PurposeExpresses codon optimized SARS-CoV-2 NSP8 in E. coliDepositorInsertSARS-CoV-2 NSP8 (ORF1ab Synthetic)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSmart-EF1a-Nla-cas7
Plasmid#178879PurposeExpression of human codon-optimized Nlacas7 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas7c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only
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