Addgene

We narrowed to 3,692 results for: c1s

Showing: 3201 - 3220 of 3692 results

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pCIBN-tagBFP-hPMLIII

Plasmid

#103807

Purpose

BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hPMLIII (CIB1, PML Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mEos3.2trLAT-2P

Plasmid

#203748

Purpose

Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLAToC27AC30A (Lat Mouse)

Use

Tags

ER translocation signal - mEos3.2

Expression

Mammalian

Mutation

C27A, C30A

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-CREB (S133A)

Plasmid

#137002

Purpose

mEGFP-CREB donor for G-CREB with S133A mutation

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Changes Serine 133 into Alanine

Promoter

CMV

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-BICD2*-strep

Plasmid

#120168

Purpose

Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

BICD cargo adaptor 2 (Bicd2 Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated BICD2: 15-595 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2B

Plasmid

#202734

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (PIP4K2B, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mEmerald-MyosinIIA-C-18

Plasmid

#54190

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TIA1 RRM-YFP-SspBx6

Plasmid

#103781

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-YFP-SspBx6

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-GLT1b-V5

Plasmid

#162518

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1b (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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SSpB-HaloTag-p63DH

Plasmid

#176116

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-TetR

Plasmid

#103797

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-TetR (CIB1, tetR E. coli, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-ThymosinBeta4(WT)

Plasmid

#96806

Purpose

cilia-membrane-targeted G-actin-binding protein

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Actin-C-18

Plasmid

#53978

Purpose

Localization: Actin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Actin (ACTB Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
CMV-mCherry-KIX-mCherry

Plasmid

#137004

Purpose

mCherry-KIX acceptor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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mRuby2-Tubulin-C-18

Plasmid

#55915

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FLAG-TPD52

Plasmid

#172452

Purpose

Expression of Tumor Protein D52 (TPD52) with N-terminal FLAG tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mMaroon-KIX-mMaroon

Plasmid

#137005

Purpose

mMaroon-KIX acceptor for R-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

Tags

mMaroon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinE442Q

Plasmid

#134464

Purpose

M1 SpastinE442Q expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Glu to Gln at amino acid 442

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR wt (Shank3)

Plasmid

#175254

Purpose

SPN-ARR fragment in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-PSPC1-CT

Plasmid

#124374

Purpose

expresses EGFP-PSPC1-CT131 in mammalian cell

Depositor

Yuh-Shan Jou

Article

Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

Insert

PSPC1 CT (PSPC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-H2B-C-10

Plasmid

#57804

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Use

Tags

tdminiSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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