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Plasmid#180524PurposedCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assemblyDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only
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KTK_324
Plasmid#180566PurposeSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2aDepositorInsertSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-spTorA-GFP(C)
Plasmid#169041PurposeGFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.DepositorInsertH6-spTorA-GFP
UseTags6xHis, TEV cleavage site, and spTorA (signal pept…ExpressionBacterialMutationThe mut3 GFP variant with 5 additional mutations …PromoteraraBADAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(Δ3)-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154169Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(Δ2)-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154170Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(Δ1)-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154171Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154172Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP-squirrel-monkey-CHMP3
Plasmid#154182Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsFLAG, Strep-Tag II, and preScission siteExpressionMammalianMutationPromoterCAGAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP-squirrel-monkey-retroCHMP3
Plasmid#154184Purposeexpresses squirrel monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsFLAG, Strep-Tag II, and preScission siteExpressionMammalianMutationPromoterCAGAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG
Plasmid#132686PurposeAAV plasmid encoding mouse ACY1 with C-terminal flag tagDepositorUseAAVTagsFlagExpressionMutationPromoterChicken b-actinAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP
Plasmid#108486Purposeinducible expression of vsv-Mis12-dCEN-INCENP-EGFPDepositorInsertCenpB-dCEN-INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationCEN box replaced by CenpB 1-166PromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-Incenp 8KQ
Plasmid#108501Purposeexpression of vsv-INCENP 8KQDepositorInsertINCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationK548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-dCEN-Incenp
Plasmid#108479Purposeexpression of vsv-dCEN-INCENPDepositorInsertdCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationCEN box removedPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
HASP_HUMAN_D0
Plasmid#79730PurposeThis plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertHASP
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC2D_HUMAN_D0
Plasmid#79734PurposeThis plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2D
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAPK2_HUMAN_D0
Plasmid#79738PurposeThis plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertAAPK2
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC4_HUMAN_D0
Plasmid#79744PurposeThis plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC4 (SLC12A7 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC1G_HUMAN_D0
Plasmid#79745PurposeThis plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC1G
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC2A_HUMAN_D0
Plasmid#79746PurposeThis plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2A
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1G2_HUMAN_D0
Plasmid#79716PurposeThis plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1G2
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only