Addgene

We narrowed to 5,035 results for: pcdna3.1

Showing: 3181 - 3200 of 5035 results

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human-GFAP-EGFP

Plasmid

#169473

Purpose

We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

EGFP-hGFAP (GFAP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Article

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pANAP

Plasmid

#48696

Purpose

allows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

Depositor

Peter Schultz

Article

Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

Inserts

8 copies of the amber suppressor tRNACUA-Leu
AnapRS

Use

Tags

Expression

Mammalian

Mutation

mutant E.coli leucyl synthetase specific for Anap…

Promoter

CMV and human H1 promoter

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pFH2.214_mCherry-PP7x12

Plasmid

#205556

Purpose

Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-EGFP-RIP2

Plasmid

#131201

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 (RIPK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Mfn2-Myc

Plasmid

#23213

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn2 (Mfn2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1

Plasmid

#1026

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(Hygro) myc-hPolQ-Flag

Plasmid

#73132

Purpose

To express in mammalian cells human PolQ

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

Polymerase theta (POLQ Human)

Use

Tags

Flag and myc

Expression

Mammalian

Mutation

Promoter

cmv promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-MRGBP

Plasmid

#15325

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

Insert

MRG-binding protein (MRGBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
Red cGull

Plasmid

#191853

Purpose

Expresses biosensor for cGMP in mammalian cells

Depositor

Takashi Tsuboi

Article

Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

Insert

Red cGull

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.10_Gag-MCP-P2A-eGFP

Plasmid

#205525

Purpose

Express HIV Gag-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-CytERM-mCherry-(F30-2xPepper)10

Plasmid

#129403

Purpose

CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

CytERM-mCherry-(F30-2xPepper)10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-Sumo2 WT

Plasmid

#48967

Purpose

Expresses HA SUMO2 Wild Type in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO2 (SUMO2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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eTC GFP beta-actin full length

Plasmid

#27123

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Use

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

E237A

Promoter

Available since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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LgBit-NbALFA

Plasmid

#201484

Purpose

NanoBit detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbALFA

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA Set9 wt

Plasmid

#24084

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

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miniCMV-tdTomato-tDeg

Plasmid

#217521

Purpose

Expresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoter

Depositor

Xing Li

Article

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

tdTomato-tDeg fluorogenic protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

miniCMV

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDV10_pCMV-ADAR1-p150

Plasmid

#200405

Purpose

Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

ADAR-p150

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Insert

Src biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pC0078 RESCUE

Plasmid

#130661

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd (RESCUE)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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