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Plasmid#218590PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationErg20(F96W, N127W), Erg20-Erg20 synthetic homodim…PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-MpPHOT
Plasmid#220098PurposeTransient expression of MpPHOT in plant cellsDepositorInsertMpPHOT
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AePHOT
Plasmid#220097PurposeTransient expression of AePHOT in plant cellsDepositorInsertAePHOT
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-3Ty
Plasmid#221047PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xTy epitopes
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTvGPI(EP)-blast-mNG
Plasmid#221051PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-3HA
Plasmid#221048PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xHA epitopes
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-3Myc
Plasmid#221046PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xMyc epitopes
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-3FLAG
Plasmid#221045PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xFLAG epitopes
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-blast-His:mNG:His
Plasmid#201094PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-hygro-His:mNG:His
Plasmid#201096PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS2-1
Plasmid#220627PurposePlasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_3ABC_C147R
Plasmid#212985PurposeEncodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus Type I Mahoney 3ABC C147R
UseMammalian cell free protein expressionTagsHAExpressionMutationcysteine 147 in 3C changed to argininePromoterT7Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_EVA71_VP12A_C110A
Plasmid#212976PurposeEncodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertEnterovirus A71 4643 VP12A C110A
UseMammalian cell free protein expressionTagsHA tagExpressionMutationchanged cysteine 110 from 2A to alaninePromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_VP12A_C109R
Plasmid#212987PurposeEncodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus Type I Mahoney VP12A C109R
UseMammalian cell free protein expressionTagsHAExpressionMutationCysteine 109 from 2A mutated to argininePromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-moxNG
Plasmid#190222PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmoxNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pIA028
Plasmid#200419PurposethrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloningDepositorArticleTypeEmpty backboneUseTagsPAmCherry1ExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-Ppyr9eh
Plasmid#201443PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertPpyr9eh
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mNG:Ppyr9eh
Plasmid#201441PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG:Ppyr9eh
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-g418-His:mScarletI:His
Plasmid#201098PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet-I
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only