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Plasmid#165911PurposeBlasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1258
Plasmid#44478DepositorInsertMinimal Mos1, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceApril 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only
Plasmid#69907PurposeExpresses a circular RNA containing the 5' half of GFP in mammalian cellsDepositorInsertGFP, ZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1273
Plasmid#44487DepositorInsertMinimal Mos1, peel-1, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
UseTagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Omega2
Plasmid#165865PurposeOmega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRB20
Plasmid#52559Purposeserves as PCR-template to generate HR donors for C-terminal YFP-tagDepositorInserteYFP and Blasticigin resistance cassette
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC+CKK
Plasmid#59048Purposefor baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)DepositorInsertPatronin (Patronin Fly)
UseTagseGFPExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC 534–1457
Plasmid#59050Purposefor baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)DepositorInsertPatronin (Patronin Fly)
UseTagseGFPExpressionBacterial and InsectMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Khc73-mCherry
Plasmid#31752DepositorInsertKhc73 (Khc-73 Fly)
UseTagsmCherryExpressionMammalianMutationPromoterMetallothioneinAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR[traF]+Opie2-PuroR[dsxM]
Plasmid#131618PurposeExpresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.DepositorInsertsPuroR[dsxM]
NeoR[traF]
UseTagsExpressionInsectMutationPromoterOpie2Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T
Plasmid#79261PurposeExpresses EGFP-tagged Dm4E-T in S2 cellsDepositorInsertDm4E-T (4E-T Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pattB-3xFlag-Loqs-PD
Plasmid#41110DepositorInsertLoquacious-PD (loqs Fly)
UseTags3xFlagExpressionInsectMutationPromoterAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1417 (No intron)
Plasmid#69904PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in mammalian cellsDepositorInsertZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationF169L in ZKSCAN1PromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-EBFP2-MiniWhite
Plasmid#165918PurposeBlasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot2
Plasmid#79255PurposeExpresses EGFP-tagged DmNot2 in S2 cellsDepositorInsertDmNot2 (Rga Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3
Plasmid#79250PurposeExpresses EGFP-tagged DmNot3 in S2 cellsDepositorInsertDmNot3 (Not3 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato fascin WT
Plasmid#53508DepositorInsertfascin (sn Fly)
UseTagstdtomatoExpressionInsectMutationPromoterGAL4Available sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)
Plasmid#126026Purposeexpress RFP-tagged LARIAT variant for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHRolig-VHH(GFP)
UseTagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1
Plasmid#86381PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster zfh1 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only