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Plasmid#184419PurposeTransformation of mitochondrial DNA in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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pFM699
Plasmid#74636Purposealias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection markerDepositorInsertmCherry
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBD-pZ-FLAG
Plasmid#183148PurposeYeast-two-hybrid BD vector with pZ promoter (activated by ZPM)DepositorInsertGAL4 BD
UseYeast two-hybridTagsFLAGExpressionYeastMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIM691
Plasmid#74641Purposealias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#162606PurposePre-express Cas9 in yeastDepositorInsertTef1-Cas9
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ374
Plasmid#59896PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCY 3090-06
Plasmid#36233DepositorInsertURA3
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-mCherry-URA3ExpressionMutationPromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIM573
Plasmid#74637Purposealias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM701
Plasmid#74642Purposealias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM700
Plasmid#74639Purposealias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAH237
Plasmid#121146PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeastDepositorInsertsgRNA cassette
humanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoterS.pombe rrk1 and nmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFM689
Plasmid#74635Purposealias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection markerDepositorInsertsUseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM690
Plasmid#74638Purposealias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM572
Plasmid#74640Purposealias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZCAG
Plasmid#129114PurposeZ. rouxii centromeric vector containing G418 resistanceDepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZEG
Plasmid#129112PurposeZ. rouxii episomal vector containing G418 resistanceDepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCY 3010-05
Plasmid#36211DepositorInsertLEU2
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-Cerulean-LEU2ExpressionMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3040-05
Plasmid#36220DepositorInsertLEU2
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEGFP-LEU2ExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-04
Plasmid#36228DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-TRP1ExpressionMutationPromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only