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Plasmid#65680PurposeExpresses beta-tubulin-ONL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
1114H
Plasmid#200640PurposeAn. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile malesDepositorInsertgRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pDK616
Plasmid#134202PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD) mutantDepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationK223A K226APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJS263
Plasmid#134141PurposeBacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)DepositorInsertSka1 MTBD-His (Y106G6H.15 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW087
Plasmid#134128PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationK183A K184APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW090
Plasmid#134129PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationK223A K226APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW070
Plasmid#134138PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationresidues 1-131, CRISPR resistantPromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW093
Plasmid#134137PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R155APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW082
Plasmid#134136PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R236APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW088
Plasmid#134135PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, K223A K226APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW085
Plasmid#134134PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, K183A K184APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-RFP SADBs
Plasmid#67153PurposepSG5 containing N-terminally RFP tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsRFPExpressionMammalianMutationPromoterSV40Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-HA SADBs
Plasmid#67149PurposepSG5 containing N terminally HA tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterSV40, T7Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_TUBB5
Plasmid#65679PurposeExpresses beta-tubulin-CNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSS-SGFP2-aTub
Plasmid#112936PurposeVisualization of microtubulesDepositorInsertalpha tubulin
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1[D157N]
Plasmid#27100DepositorInsertαTubulin K40 acetyltransferase (ATAT1 Human)
UseTagsGFP and S-tagExpressionMammalianMutationD157NPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pET21 E2F1
Plasmid#90313PurposeExpresses human E2F1 in bacteriaDepositorInsertE2F1 (E2F1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits