Addgene

We narrowed to 1,160 results for: smo

Showing: 301 - 320 of 1160 results

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pSL1103

Plasmid

#129526

Purpose

pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Expression

Mutation

Promoter

Available since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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36INS-J23100_FB

Plasmid

#78668

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-J23100_AB

Plasmid

#78666

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSL1315

Plasmid

#129527

Purpose

pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Expression

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSL1360

Plasmid

#219777

Purpose

pDEF-TurboID-GFPmut2, empty vector for creating C-terminal fusion proteins.

Depositor

Scott Lindner

Article

Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

Insert

TurboID/GFPmut2

Use

Plasmodium, rodent malaria parasites

Tags

Expression

Mutation

Promoter

None

Available since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1419

Plasmid

#219778

Purpose

pDEF-TurboID-GFPmut2, negative control for cytosolic expression

Depositor

Scott Lindner

Article

Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

Insert

TurboID/GFPmut2

Use

Plasmodium, rodent malaria parasites

Tags

Expression

Mutation

Promoter

PbEF1alpha promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xVSVG-GSK3(1-97)

Plasmid

#129582

Purpose

Encodes VSVG tagged GSK3(1-97) for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

GSK3a(1-97) (GSK3A Human)

Use

Tags

GST and VSVG

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET-29b-3xVSVG-GSK3(1-97)

Plasmid

#129583

Purpose

Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

GSK3a(1-97) (GSK3A Human)

Use

Tags

6xHistidine

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1211

Plasmid

#129521

Purpose

pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFlag-NLS

Expression

Mutation

Promoter

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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36INS-pTet_GB

Plasmid

#78681

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [G:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-pTet_FB

Plasmid

#78680

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [F:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-pTet_EB

Plasmid

#78679

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [E:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-pTet_AB

Plasmid

#78678

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [A:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_GB

Plasmid

#78673

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [G:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_FB

Plasmid

#78672

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [F:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_EB

Plasmid

#78671

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Article

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Use

Tags

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Promoter

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-NFLAG TRF1

Plasmid

#16058

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

aa2-439

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaM

Plasmid

#16064

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted myb domain (aa379-439)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaA

Plasmid

#16063

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted acidic domain (aa1-66)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only