Skip to main content
Addgene
Showing: 301 - 320 of 1677 results
  1. pDD2601

    Plasmid
    #175085
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S47A, S65A, T75A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pXPR_071

    Plasmid
    #164558
    Purpose
    Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pXPR_070

    Plasmid
    #164265
    Purpose
    Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Insert
    sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. Linked Free NES

    Plasmid
    #182485
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. Linked Free PcPTS

    Plasmid
    #182486
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Insert
    FPPS-PTS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. Linked Free ClLIS1 (M)

    Plasmid
    #182487
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Insert
    FPPS(M)-LS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pXPR_068

    Plasmid
    #164555
    Purpose
    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. Coexpressed Free PcPTS

    Plasmid
    #182483
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Inserts
    FPPS
    PTS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. Linked Free NES (AG4TGGA)2

    Plasmid
    #182488
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. Linked Free (PT)4P

    Plasmid
    #182490
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA4/TO-ORF66 C423A-2xStrep

    Plasmid
    #131111
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 423 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 301 - 320 of 1677 results