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Plasmid#32956PurposeFor visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.DepositorInsertATF6 (ATF6 Human)
UseTagsEGFPExpressionMammalianMutationS1Protease site mutation: R415A/R416APromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iEscSnFR_PM
Plasmid#182813PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iEscSnFR_cyto
Plasmid#182814PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_cyto
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iFluoxSnFR_ER
Plasmid#182815PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iFluoxSnFR_PM
Plasmid#182816PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iFluoxSnFR_cyto
Plasmid#182817PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_cyto
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iEscSnFR_ER
Plasmid#182818PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_ER
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iEscSnFR_PM
Plasmid#182819PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_PM
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iEscSnFR_cyto
Plasmid#182820PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_cyto
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iFluoxSnFR_ER
Plasmid#182821PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_ER
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iFluoxSnFR_PM
Plasmid#182822PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_PM
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iFluoxSnFR_cyto
Plasmid#182823PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_cyto
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iEscSnFR_ER
Plasmid#182812PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES
Plasmid#138299PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub(T/A)-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag
Plasmid#185681PurposeLow-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutationDeletion of lumenal domainPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNK7185
Plasmid#219765PurposeVector for expression PzPKS2 in yeast (P.pastoris)DepositorInsertPzPKS2
UseTagsExpressionYeastMutationPromoterpGAPAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7184
Plasmid#219756PurposeVector for expression PzPKS2 in mammalian cells (HEK293NT)DepositorInsertPzPKS2
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7183
Plasmid#219760PurposeMoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)DepositorInsertPzPKS2
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pBabe-EGFP-Hu_Plk1(WT)
Plasmid#136323PurposeRetroviral expression of human Plk1-WT with N-terminal EGFPDepositorInsertPLK1 (PLK1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only