-
Plasmid#196714PurposeWT SpCas9 with BSD-TagBFP for selection and sortingDepositorInsertCas9-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPRv2-sgEGFP
Plasmid#86153PurposeLentivirus carrying Cas9/CRISPR for cut in GFP (used as control)DepositorInsertEGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-ATG5
Plasmid#99573PurposeExpresses Cas9 with sgRNA targeting Exon 7 of ATG5DepositorInsertgRNA targeting ATG5 (ATG5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HIFR
Plasmid#192946PurposeLentiviral production of HIF-1alpha reporterDepositorInsertHIF-1alpha reporter
UseLentiviralTagsExpressionMutationPromotermCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_CjCas9-Puro
Plasmid#202564PurposehU6-BsmBI-gRNA-PuroDepositorInsertCjCas9 sgRNA cassette
UseTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-mCherry
Plasmid#75161PurposeDerived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Crimson
Plasmid#70683PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Crimson Reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti.pFOXA2.GFP
Plasmid#59404Purposeexpresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during developmentDepositorInsert400 bp FOXA2 promoter, eGFP (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpFOXA2Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(PX-LD)
Plasmid#214422PurposeDetection of peroxisome-lipid droplet contact siteDepositorInsertPMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)
UseLentiviralTagsCFAST-Halo and FKBP-V5-RspANExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-hygro
Plasmid#160090PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-STING_gRNA3
Plasmid#127640PurposeKnock-out of human STINGDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A3 WT
Plasmid#195849PurposeExpresses wild-type Homo sapiens ATP13A3DepositorInsertATPase 13A3 (ATP13A3 Human)
UseLentiviralTagsExpressionMutationD498N, L956P, M850I, V855M, R858H, L675VPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MP2
Plasmid#36097Purpose3rd generation lentiviral plasmidDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cdc42-2G
Plasmid#68813PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)DepositorInsertCdc42 activation reporter
UseLentiviralTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW13.lentiguide.mCherry
Plasmid#104375PurposemCherry vector for cloning in guidesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Cherry
Plasmid#170510PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TagGFP2
Plasmid#121426PurposeTAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.DepositorInsertempty TagGFP2
UseLentiviralTagsTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SPA2G231V
Plasmid#42079DepositorInsertSurfactant Protein -A2 (SFTPA2 Human)
UseLentiviral; PlentiTagsV5ExpressionBacterial and MammalianMutationChanged Glycine 231 to ValinePromoterCMVAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-GFP
Plasmid#35637DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -