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Showing: 301 - 320 of 926 results

  8. cr3Vn

    Plasmid
    #184861
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, gam, bet, exo
    Use
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184861-plasmid-map-sequence-id-363183

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  10. pKB01_mCherry

    Plasmid
    #69104
    Purpose
    Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.
    Depositor
    Jan-Willem Veening
    Article
    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69104-plasmid-map-sequence-id-125508

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  12. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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  13. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Ptet
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

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  16. cr3Ec

    Plasmid
    #184860
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, gam, bet, exo
    Use
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184860-plasmid-map-sequence-id-363182

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Showing: 301 - 320 of 926 results