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Showing: 301 - 320 of 12090 results

  1. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203652
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203652-plasmid-map-sequence-id-402483

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  4. pNUT3

    Plasmid
    #175744
    Purpose
    Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3
    Depositor
    Yubin Zhou
    Article
    Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655
    Insert
    mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    175744-plasmid-map-sequence-id-361253

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  5. pNUT2

    Plasmid
    #175745
    Purpose
    Single-component photo-inducible (LOV2-based) nucleolus-targeting tool 2
    Depositor
    Yubin Zhou
    Article
    Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655
    Insert
    mCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    175745-plasmid-map-sequence-id-365725

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  13. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid
    #84730
    Purpose
    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Insert
    HyPer2 targeted to nucleus
    Use
    Tags
    NLS (nuclear localisation signal and NLS (nuclear…
    Expression
    Plant
    Mutation
    Promoter
    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84730-plasmid-map-sequence-id-180307

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  14. pEndo-I-OnuI-TSM

    Plasmid
    #207954
    Purpose
    Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Insert
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in fu…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207954-plasmid-map-sequence-id-412663

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  16. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    FKBP-ICUE3-NLS
    Use
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    138255-plasmid-map-sequence-id-307462

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  19. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Article
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Mutation
    Promoter
    RPBSA and TRE promoter
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

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Showing: 301 - 320 of 12090 results