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Showing: 301 - 320 of 425 results

  2. pDBD-M

    Plasmid
    #50572
    Purpose
    Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal1
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Use
    Tags
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Article
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Use
    Tags
    Expression
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

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  14. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    DHFR P66L
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

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  15. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Article
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Insert
    DD-dCas9VP192-T2A-EGFP
    Use
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69534-plasmid-map-sequence-id-122831

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  16. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Tags
    Expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  19. pDonorTp

    Plasmid
    #211788
    Purpose
    pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR
    Depositor
    Silvia Cardona
    Insert
    dhfr
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211788-plasmid-map-sequence-id-418339

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  20. pSuccessor

    Plasmid
    #228391
    Purpose
    intended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithography
    Depositor
    Ingmar Riedel-Kruse
    Insert
    pConst23100-sfGFP__pLambda-DHFR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228391-plasmid-map-sequence-id-453567

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Showing: 301 - 320 of 425 results