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C321.∆A ompT- rne- lon-

Bacterial Strain

#182773

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
pIRES2_EGFP_KCNQ1

Plasmid

#173161

Purpose

For mammalian expression of KCNQ1 and creating variants

Depositor

Al George

Article

Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

Insert

KCNQ1 (KCNQ1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSR58.6

Plasmid

#63176

Purpose

Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaR
sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b-PunyEEER

Plasmid

#208322

Purpose

Bacterial expression of the His-tagged peptide PunyEEER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PunyERRE

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TREER

Plasmid

#208321

Purpose

Bacterial expression of the His-tagged peptide TREER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TinyERRE

Plasmid

#208320

Purpose

Bacterial expression of the His-tagged peptide TinyERRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TinyERRE

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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FR-E01

Bacterial Strain

#118727

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Bacterial Resistance

None

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only
sfCherry11-WT-PTPN22

Plasmid

#195384

Purpose

Mammalian expression of human wild-type PTPN22 with sfCherry(11) tag

Depositor

Patrick Concannon

Insert

PTPN22 (PTPN22 Human)

Use

Tags

sfCherry(11)

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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sfCherry11-620W-PTPN22

Plasmid

#195386

Purpose

Mammalian expression of a human PTPN22 mutant (620W) with sfCherry(11) tag

Depositor

Patrick Concannon

Insert

PTPN22 (PTPN22 Human)

Use

Tags

sfCherry(11)

Expression

Mammalian

Mutation

R620W (rs2476601)

Promoter

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUC118

Plasmid

#50009

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Vieira et al Methods Enzymol. 1987;153:3-11.

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pUC119

Plasmid

#50010

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Vieira et al Methods Enzymol. 1987;153:3-11.

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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WT-short-UBASH3A-GFP11

Plasmid

#195385

Purpose

Mammalian expression of human wild-type short-form UBASH3A with GFP(11) tag

Depositor

Patrick Concannon

Insert

UBASH3A (UBASH3A Human)

Use

Tags

GFP(11)

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-SUZ12 HDR - pDY082

Plasmid

#171110

Purpose

Homology directed repair plasmid for generating HaloTag-SUZ12 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

SUZ12 (SUZ12 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSR59.4

Plasmid

#63175

Purpose

Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

OmpR (ompR )
sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Puromycin-mVenus-MAP

Plasmid

#44118

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

mVenus-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Hygromycin-sfGFP-MAP

Plasmid

#44100

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

sfGFP-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Hygromycin-mVenus-MAP

Plasmid

#44099

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

mVenus-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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