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Plasmid#223712PurposeExpresses autophagy flux LC3B fluorescence reporterDepositorInsertLC3B (MAP1LC3B Human, Synthetic)
UseLentiviral and Synthetic BiologyTagsmCherry::EGFP::LC3BExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE
Plasmid#138151PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR_BsmBI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73037PurposeExpress Nluc and copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE
Plasmid#125256Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selectionDepositorInserthChR2(H134R)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1a-forwardAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
Plasmid#141385PurposeLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
Plasmid#141386PurposeLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
Plasmid#141391PurposeLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}
Plasmid#201120PurposeRFP expression in cells under EF-1α promoter; Histone-bound, nuclearDepositorInsertTurboRFP; H2B-RFP
UseLentiviralTagsExpressionMutationNoPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1
Plasmid#118593PurposeExpresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoterDepositorInsertPuro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha/TRE3GAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227APromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE
Plasmid#101104PurposeEncodes dCas9(N) fused to VEGFR2DepositorInsertVEGFR2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro
Plasmid#216244PurposeExpress AtAFB2.F74A mutant with mCherry tag for AID2DepositorInsertAtAFB2.F74A-mCherry
UseCRISPR, TALEN, and Unspecified ; Human safe harbo…TagsmCherryExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)
Plasmid#214876PurposeLentiviral vector encoding RfxCas13d nontargeting control guideDepositorInserthU6-crNT-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-DIO-Nluc-copGFP-IRES-Puro
Plasmid#192875PurposeCre-dependent dual reporterDepositorInsertNanoluc-P2A-copGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc)
Plasmid#160368PurposeStable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURODepositorInsertGPR143 (GPR143 Human)
UseLentiviralTagsmScarlet-mycExpressionMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro
Plasmid#89991PurposeDonor template for generation of SOX2-ntdTomato reporter cell linesDepositorInsertSOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro
Plasmid#210604Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro
Plasmid#170320PurposeExpresses HA and mCherry tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro
Plasmid#170317PurposeExpresses HA and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only