Addgene

We narrowed to 5,017 results for: hwa

Showing: 3161 - 3180 of 5017 results

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pGL4_10xUAS-CMVmin-BC0442-luc2

Plasmid

#227130

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0442

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0442 and a luciferase reporter gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-shNC-mCherry

Plasmid

#222962

Purpose

Negative control for lentiviral shRNA

Depositor

Xinyu Zhao

Article

Guo et al Nat Commun. 2023 Jun 26;14(1):3801. doi:

Insert

Negative control

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD80-CLIP

Plasmid

#223590

Purpose

Lentiviral expression of human CD80 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD80

Plasmid

#223588

Purpose

Lentiviral expression of human CD80 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg1

Plasmid

#198566

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg1 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg2

Plasmid

#198567

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg2 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-IRAK4-mScarlet-P2A-Puro

Plasmid

#221237

Purpose

Stable expression of mouse IRAK4-mScarlet after Lentiviral transduction

Depositor

Jonathan Kagan

Article

Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

Insert

IRAK4 (Irak4 Mouse)

Use

Lentiviral

Tags

mScarlet

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-K17R-K22R

Plasmid

#220294

Purpose

mammalian expression of Bcl-2-K17R-K22R tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Use

Tags

Emerald GFP

Expression

Mammalian

Mutation

K17R-K22R

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-G128L-A131L-T132L

Plasmid

#220304

Purpose

mammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Use

Tags

Emerald GFP

Expression

Mammalian

Mutation

G128L-A131L-T132L

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 wt

Plasmid

#210266

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Use

Tags

mCherry

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 wt

Plasmid

#210262

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PRPF4-1

Plasmid

#156158

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF4 (PRPF4 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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sgVRK1-hygro

Plasmid

#199640

Purpose

sgRNA guide against VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-EBM1-2mut-siRNAres_K

Plasmid

#146751

Purpose

Mammalian Expression of HsSmg6-EBM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-EBM1-2mut-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent and two non silent R291P, N341T mutati…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-EBM1mut-siRNAres_K

Plasmid

#146752

Purpose

Mammalian Expression of HsSmg6-EBM1mut-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-EBM1mut-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-EBM2mut-siRNAres_K

Plasmid

#146753

Purpose

Mammalian Expression of HsSmg6-EBM2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-EBM2mut-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIDRB86-EBMmut_K

Plasmid

#146762

Purpose

Mammalian Expression of HsGIDRB86-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsGIDRB86-EBMmut (C10orf28 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIDRP86_K

Plasmid

#146763

Purpose

Mammalian Expression of HsGIDRP86

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsGIDRP86 (C10orf28 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsR3HCC1_39-480-EBMmut_K

Plasmid

#146765

Purpose

Mammalian Expression of HsR3HCC1_39-480-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsR3HCC1_39-480-EBMmut (R3HCC1 Human)

Use

Tags

Expression

Mammalian

Mutation

sequence extracted from BC050572.1; 39-480 trunca…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-del42-58-siRNAres_K

Plasmid

#146749

Purpose

Mammalian Expression of HsSmg6-del42-58-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del42-58-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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