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Plasmid#136991PurposeTo express a protein of interest fused to the C-terminus of Flag-2xAPEX2 for proximity labeling or TEMDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCAGGS-SARS2-S-FKO (NC-flag)
Plasmid#159363PurposeMammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.DepositorInsertSARS-CoV-2 S protein (S SARS-CoV-2)
UseTagsFlag and FlagExpressionMammalianMutationFurin-cleavage site mutationPromoterCMVAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 FLAG-BMP2-Fc
Plasmid#115771PurposeExpression of the extracellular domain of BMP2 with N-terminal FLAG tag and C-terminal hIgG tag.DepositorInsertBMP2 (BMP2 Human)
UseTagsFLAG and hIgG/FcExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-3xFLAG-NCOA4 (dIDR1)
Plasmid#192809PurposeStable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cellsDepositorInsertNCOA4 (NCOA4 Human)
UseTags3xFLAGExpressionMammalianMutationDeletion of the IDR1PromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B Flag BimS
Plasmid#23283DepositorInsertpCMV Tag2b Bim S (Bcl2l11 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 FS10
Plasmid#113956Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, S212N substitution…PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Flag-TTGZFP-FokI-DD
Plasmid#18755DepositorInsertzinc finger nuclease
UseTagsExpressionBacterialMutationRNA expression vector for expressing the zinc fin…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAP G116
Plasmid#123099PurposeExpresses 3xFLAG-GABARAP G116 in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAGExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG
Plasmid#127322PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-PVR(FL)-FLAG
Plasmid#157622PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorInsertPVR (PVR Human)
UseTags6xHis and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B11-V16D-FLAGC
Plasmid#184500PurposeExpresses the V16D mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInsert17-beta-hydroxysteroid dehydrogenase 11 (HSD17B11 Human)
UseTagsFLAGC-GFPExpressionMammalianMutationV16DPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B11-L14P-FLAGC
Plasmid#184499PurposeExpresses the L14P mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInsert17-beta-hydroxysteroid dehydrogenase 11 (HSD17B11 Human)
UseTagsFLAGC-GFPExpressionMammalianMutationL14PPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B11-S172L-FLAGC
Plasmid#161904PurposeExpresses the catalytically inactive S172L mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInserthydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
UseTagsFLAG-GFPExpressionMammalianMutationS172L; catalytically dead mutantPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag WDR5
Plasmid#15553DepositorInsertWdr5 (Wdr5 Mouse)
UseTagsFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-CSNK1E
Plasmid#38078DepositorInsertCSNK1E casein kinase 1, epsilon (CSNK1E Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pICE-EGFP-FLAG-Ku70siR-WT
Plasmid#46961PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman Ku70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMV TetAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-E112A
Plasmid#110215PurposeExpression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsFlagExpressionMammalianMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
WFS1 P724L-Flag-pcDNA3
Plasmid#13012DepositorInsertWolfram Syndrome 1 (WFS1 Human)
UseTagsFlagExpressionMammalianMutationchanged P 724 to LPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KLF12/pcDNA3.1-Neo
Plasmid#81069PurposeExpression of epitope-tagged human KLF12 in mammalian cells.DepositorInsertFLAG-KLF12 (KLF12 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only