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Showing: 3161 - 3180 of 8619 results

  3. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  4. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1-a
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  19. Double UP mNeon to mScarlet Rac1-V12

    Plasmid
    #125136
    Purpose
    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).
    Depositor
    Erik Dent
    Article
    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d
    Insert
    mNeon/mScarletP2A Rac1-V12
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Removed Not1 site in mScarlet. 12th AA in Rac1 ch…
    Promoter
    CAG
    Available since
    May 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    125136-plasmid-map-sequence-id-244760

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Showing: 3161 - 3180 of 8619 results