Addgene

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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Use

Tags

FLAG and NLS

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_H2B

Plasmid

#65702

Purpose

Expresses YNL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

Plasmid

#140087

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBDT

Plasmid

#62660

Purpose

Targeting vector for PITT landing pad knock-in into the Rosa26 locus

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

eGFP
neomycin

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pONL-N1_LAMP1

Plasmid

#65686

Purpose

Expresses LAMP1-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pONL-C1_H2B

Plasmid

#65704

Purpose

Expresses ONL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBDU

Plasmid

#62661

Purpose

Targeting vector for PITT landing pad knock-in into the Rosa26 locus

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

eGFP
neomycin

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBGV

Plasmid

#62664

Purpose

Donor vector for mCFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

mCFP

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pONL-N1_PTS2

Plasmid

#65677

Purpose

Expresses PTS2-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_H2B

Plasmid

#65703

Purpose

Expresses CNL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221_zCas9

Plasmid

#213913

Purpose

Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Ziqiang P. et al Bio Protocol, Vol 14, Iss 13, Jul 5, 202

Insert

zCas9

Use

CRISPR

Tags

Expression

Mutation

Zea mays codon-optimized Cas9

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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G1088E_pLenti-CMV-mNeonGreen-2A-HygroR

Plasmid

#216279

Purpose

Green and hygromycin resistant lentiviral vector plasmid for multiplexed receptor pseudovirus experiments

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2024 May 20;20(5):e1012044.

Insert

mNeonGreen-2A-Hpt

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN1_EF1a-tNGFR-2A-Puro

Plasmid

#200553

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 1

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN2_EF1a-tNGFR-2A-Puro

Plasmid

#201112

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 2

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN3_EF1a-tNGFR-2A-Puro

Plasmid

#201113

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 3

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN4_EF1a-tNGFR-2A-Puro

Plasmid

#201114

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 4

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN5_EF1a-tNGFR-2A-Puro

Plasmid

#201115

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 5

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN6_EF1a-tNGFR-2A-Puro

Plasmid

#201116

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 6

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN7_EF1a-tNGFR-2A-Puro

Plasmid

#201117

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 7

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN8_EF1a-tNGFR-2A-Puro

Plasmid

#201118

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 8

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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