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Showing: 3141 - 3160 of 3967 results

  2. pFrt-invCAG-Luc

    Plasmid
    #63577
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63577-plasmid-map-sequence-id-105728

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  17. LUC2 (L/Sec/deltaP)

    Plasmid
    #112145
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and d…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112145-plasmid-map-sequence-id-214904

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  18. LUC45 (L/Sec/mutP)

    Plasmid
    #112148
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and SE…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112148-plasmid-map-sequence-id-214874

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  20. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Availability
    Academic Institutions and Nonprofits only
    123363-plasmid-map-sequence-id-240469

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Showing: 3141 - 3160 of 3967 results