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Plasmid#52974Purposeinsect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196A LKB1 phosphorylation defective mutantPromoterHsp70Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT006
Plasmid#182716PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloningDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsGFP in cloning site of crRNA for easier screening…ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWKS11
Plasmid#139418PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SCARF-bio-His
Plasmid#51826PurposeExpresses full-length Endothelial cells scavenger receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSCARF (SCARF1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ths pUAST-HA
Plasmid#69769PurposeThisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertThisbe (FGF8like-1)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationEncodes amino acids 1-739 of Thisbe (NP_610701.2)…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-MΔN
Plasmid#31624DepositorInsertClaspin (CLSPN Human)
UseTagsGSTExpressionBacterialMutationaa 899-1206 onlyPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV Gsk3 sgRNA/GFP
Plasmid#112733PurposeGsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.DepositorInsertGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERPINE2-bio-His
Plasmid#53416PurposeExpresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSERPINE2 (SERPINE2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129G
Plasmid#36049DepositorInsertalpha-synuclein S129G (SNCA Human)
UseTagsExpressionBacterialMutationS129GPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
Dynamin-I-K44A-6His/Flag
Plasmid#32696DepositorInsertDynamin I (Dnm1 Rat)
UseTagsFlag and His6ExpressionMammalianMutationK44APromoterCMVAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
shNg Lenti FHRC3J1UGW
Plasmid#92233Purposelentiviral expression of EGFP and Ng shRNADepositorInsertshRNA targeting Ng
UseLentiviralTagsExpressionMammalianMutationPromoterH1Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRAF4
Plasmid#58318PurposeEGFP-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pclbw-PAGFPomp25
Plasmid#58427PurposeMammalian expression of photoactivatable GFP targeted to the mitochondrial outer membraneDepositorInsertpaGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsPAGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25Promotercmv-bactinAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3
Plasmid#121976Purposetransfection studies in eukaryotic cells such as COS-7 cellsDepositorInsertType I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCIG-Flag-Sox9-IRES-nls-GFP
Plasmid#44281DepositorInsertFlag-Sox9 (SOX9 Chicken)
UseTagsFlagExpressionMammalianMutationPromoterCMV-IE enhancer and Chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
SERPINC1-bio-His
Plasmid#52076PurposeExpresses full-length Antithrombin-III precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSERPINC1 (SERPINC1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPPAT-2XStrep
Plasmid#125620Purposeexpresses human PPAT protein with a C-terminal 2XStrep affinity tagDepositorInsertPPAT (PPAT Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only