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Plasmid#197941PurposeExpression of CoA-Snifit in bacterial cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionBacterialMutationPanK=PanK-V97T, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K403A
Plasmid#195612PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationA1463G, A1464CPromoterT7Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K223I
Plasmid#195615PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activityDepositorInsertI-SceI
UseTagsExpressionBacterialMutationC2694G, G2345C, G2351C, A3365T, A3366TPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K122I
Plasmid#195614PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activityDepositorInsertI-SceI
UseTagsExpressionBacterialMutationC2458G, A2826T, A2827TPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC D218A
Plasmid#195608PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT928C, T929C, A942CPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K301A
Plasmid#195611PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationA822G, A823C, A824TPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2_506-938-H609A_O
Plasmid#147083PurposeBacterial Expression of NcQde-2_506-938-H609ADepositorInsertNcQde-2_506-938-H609A
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2_506-938-T642E_O
Plasmid#147084PurposeBacterial Expression of NcQde-2_506-938-T642EDepositorInsertNcQde-2_506-938-T642E
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41P-DmGW182_635-861_N
Plasmid#147014PurposeBacterial Expression of DmGW182_635-861DepositorInsertDmGW182_635-861 (gw Fly)
UseTagsExpressionBacterialMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41P-DmGW182_700-840_N
Plasmid#147015PurposeBacterial Expression of DmGW182_700-840DepositorInsertDmGW182_700-840 (gw Fly)
UseTagsExpressionBacterialMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41P-DmGW182_700-861_N
Plasmid#147016PurposeBacterial Expression of DmGW182_700-861DepositorInsertDmGW182_700-861 (gw Fly)
UseTagsExpressionBacterialMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41P-DmGW182_744-840_N
Plasmid#147017PurposeBacterial Expression of DmGW182_744-840DepositorInsertDmGW182_744-840 (gw Fly)
UseTagsExpressionBacterialMutationOne non silent mutation N799S and one silent muta…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-DmAgo1_572-984-optim_K
Plasmid#146728PurposeBacterial Expression of DmAgo1_572-984-optimDepositorInsertDmAgo1_572-984-optim (AGO1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE-2_506-646_J
Plasmid#146646PurposeBacterial Expression of NcQde-2_506-646DepositorInsertNcQde-2_506-646
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmGW182_1-539_G
Plasmid#146380PurposeBacterial Expression of DmGW182_1-539DepositorInsertDmGW182_1-539 (gw Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-P-HsRCK_296-472-mut_E
Plasmid#146172PurposeBacterial Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c(plus)-DmAGO1-RC_F
Plasmid#146262PurposeBacterial Expression of DmAGO1-RCDepositorInsertDmAGO1-RC (AGO1 Fly)
UseTagsExpressionBacterialMutationone non silent mutation R787C compared to sequenc…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c(plus)-DmGW182_1-539_F
Plasmid#146263PurposeBacterial Expression of DmGW182_1-539DepositorInsertDmGW182_1-539 (gw Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-HsRad23-UBA_F
Plasmid#146266PurposeBacterial Expression of HsRad23-UBADepositorInsertHsRad23-UBA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-HsTNRC6A-UBA_F
Plasmid#146267PurposeBacterial Expression of HsTNRC6A-UBADepositorInsertHsTNRC6A-UBA (TNRC6A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only