Addgene

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pWKS1583

Plasmid

#70188

Purpose

Modular vector for secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Actin/pcDNA3

Plasmid

#67776

Purpose

Actin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
EYFP-Sec23A

Plasmid

#66611

Purpose

YFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
Jnk3a1

Plasmid

#29577

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Jnk3a1 (MAPK10 Human)

Use

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Contains residues 40-402

Promoter

Available since

July 16, 2011

Availability

Academic Institutions and Nonprofits only
pDEST51-LRRK2-Y1699C

Plasmid

#25084

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Tyrosine 1699 mutated to Cysteine

Promoter

Available since

July 2, 2010

Availability

Academic Institutions and Nonprofits only
TLS 4: hTLS-CHOP.pBSKS(+)

Plasmid

#21830

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS-CHOP (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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TRPML1-MYC

Plasmid

#18824

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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MKK7

Plasmid

#29576

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK7 (Map2k7 Mouse)

Use

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
mTagRFP-T-Paxillin-22

Plasmid

#58024

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe-Puro-RalA-wt

Plasmid

#15251

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-D-TOPO-PAmCherry1_160-236-MCS

Plasmid

#60546

Purpose

Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 C-terminal fragment

Use

Cloning

Tags

Expression

Mutation

amino acids 160-236

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1_1-159-MCS

Plasmid

#60545

Purpose

Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 N-terminal fragment

Use

Cloning

Tags

Expression

Mutation

amino acids 1-159

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pILGFP3

Plasmid

#65451

Purpose

pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Use

Tags

yEGFP reporter

Expression

Yeast

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pITGFP3

Plasmid

#65452

Purpose

pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Use

Tags

yEGFP-CLN2PEST reporter

Expression

Yeast

Mutation

Promoter

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Use

Tags

Expression

Mammalian

Mutation

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM55351

Plasmid

#169092

Purpose

MoClo level 1 35S promoter-BvADHα-Nos terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvADHα-Nos terminator

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBS mPrx1 Cre (CT#644)

Plasmid

#13962

Depositor

Cliff Tabin

Article

Logan et al Genesis. 2002 Jun . 33(2):77-80.

Insert

Prx1 Cre (Prrx1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)

Plasmid

#70198

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-STf

Plasmid

#182008

Purpose

CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lyn11-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

Plasmid

#185686

Purpose

Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-HaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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