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Plasmid#65551PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-hsHEKsite_1_3
Plasmid#205638PurposereRNA guide targeting HEKsite_1_3DepositorInsertreRNA-HEKsite_1_3 (EPHA3 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGhMeCP2 (pc3972)
Plasmid#212033PurposeExpresses human MeCP2 tagged N-terminal to Halo-tagDepositorInsertMeCP2 (MECP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHTN_HaloTag_KIF5B[1-560]
Plasmid#213403PurposeTruncated human kinesin-1 with HaloTag at the N-terminusDepositorInsertHaloTag-KIF5B[1-560] (KIF5B Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-749_HA-GD2-28z_CAR_TFAP4
Plasmid#207490PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-852_HA-GD2-28z_TFAP4-HA
Plasmid#207502PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-858_CD19-BBz_CAR_TFAP4
Plasmid#207503PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, CD19-BBz_CAR (TFAP4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G
Plasmid#204214PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion of DNA binding domain (delDBD)PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G
Plasmid#204191PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion of R162PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G
Plasmid#204213PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on I207MPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G
Plasmid#204211PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on Q175* (*termination)PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G
Plasmid#204210PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on R178* (*termination)PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G
Plasmid#204212PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpressionDepositorInsertNK2 homeobox 1 (NKX2-1 Human)
UseLentiviralTagsPoint mutation on I207FExpressionMammalianMutationPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-S13AI15AD30KT35A-V5His6_E
Plasmid#146219PurposeInsect Expression of DmTral-LSm-S13AI15AD30KT35ADepositorInsertDmTral-LSm-S13AI15AD30KT35A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21A-V5His6_E
Plasmid#146221PurposeInsect Expression of DmTral-LSm-R21ADepositorInsertDmTral-LSm-R21A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-S13AI15AR21A-V5His6_E
Plasmid#146222PurposeInsect Expression of DmTral-LSm-S13AI15AR21ADepositorInsertDmTral-LSm-S13AI15AR21A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2.2
Plasmid#189842PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2.3
Plasmid#189843PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only