Addgene

We narrowed to 4,610 results for: INV-E;

Showing: 3121 - 3140 of 4610 results

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pET-29b-CylB

Plasmid

#48199

Purpose

Expresses CylB (acyl carrier protein) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylB

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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Atg20-24 pST39

Plasmid

#200439

Purpose

His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Inserts

Atg20
Atg24

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMA5136

Plasmid

#184853

Purpose

A retroviral vector encoding hygromycin resistance and PhiC31 recombinase

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

PhiC31o recombinase

Use

Retroviral; Phic31 attp-attb recombination/excisi…

Tags

Expression

Mammalian

Mutation

Codon-optimized for E. coli expression

Promoter

LTR

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.23-hs737.3

Plasmid

#222475

Purpose

Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 3). Variant 3 is chr10:128569437-G-A where the information is chromosome:positionInB38-RefAllele-AltAllele.

Depositor

Tychele Turner

Article

Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

Insert

hs737 enhancer sequence (sequence containing variant 3) (LOC110120928 Human)

Use

Luciferase

Tags

Expression

Mutation

Variant 3 is chr10:128569437-G-A where the inform…

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23-hs737.2

Plasmid

#222474

Purpose

Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 2). Variant 2 is chr10:128569418-T-C where the information is chromosome:positionInB38-RefAllele-AltAllele.

Depositor

Tychele Turner

Article

Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

Insert

hs737 enhancer sequence (sequence containing variant 2) (LOC110120928 Human)

Use

Luciferase

Tags

Expression

Mutation

Variant 2 is chr10:128569418-T-C where the inform…

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP (empty vector)

Plasmid

#172604

Purpose

Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Type

Empty backbone

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWZ403

Plasmid

#208113

Purpose

mSwAP-In marker cassette 1 with mScarlet-PuroTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-PuroTK

Use

Tags

P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pWZ854

Plasmid

#208116

Purpose

mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mNeonGreen-HPRT1-Neo

Use

Tags

T2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pWZ852

Plasmid

#208114

Purpose

mSwAP-In marker cassette 1 with mScarlet-HygTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-HygTK

Use

Tags

T2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-Dronpa

Plasmid

#87709

Purpose

FKBP-tagged Dronpa.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FKBP-Dronpa

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN

Plasmid

#135485

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Dronpa-DEVD-TagRFPT

Plasmid

#87704

Purpose

Fluorescent biosensor for super-resolution imaging of caspase activity.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

Dronpa-DEVD-TagRFPT

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A3-VN

Plasmid

#135506

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*03:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*03:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A24-VN

Plasmid

#135508

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*24:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*24:02 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1-VN

Plasmid

#135502

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*01:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B15-VN

Plasmid

#135514

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*15:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*15:01 (HLA-B Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B57-VN

Plasmid

#135515

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*57:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*57:1 (HLA-B Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd (Y84C/C121S/A139C)

Plasmid

#136063

Purpose

Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Y84C/C121S/A139C

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B8-VN

Plasmid

#135511

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*08:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*08:01 (HLA-B Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUT1147-HQ858691

Plasmid

#27737

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ZmMYB2 transcription factor from AM156904 (myb2 Zea mays)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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