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Plasmid#185752PurposeLevel 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).DepositorInsertHygR2
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2341_Tier3(SB)-Puro
Plasmid#169639PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2403-Tier1-PhCMV-ZeoR
Plasmid#169495PurposeTier-1 vector encoding PhCMV-driven zeocin resistance gene ZeoR (PhCMV-ZeoR-pA).DepositorInsertPCMV-driven Sh ble
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgRNA_Ago1_5
Plasmid#172470PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertsgAgo1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgRNA_Ago1_6
Plasmid#172471PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1.DepositorInsertsgAgo1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pb mD3S4-dN
Plasmid#180252PurposeP. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.DepositorInsertD3 of P. berghei HAP2 with N-glycosylation sites mutated
UseTags6HisExpressionMammalianMutationN516T, S533N and N539QPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B S395E
Plasmid#176006PurposeExpresses WIPI2B (S395E) fused to Halo in mammalian cellsDepositorInsertWIPI2B (S395E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationSerine 395 to GlutamatePromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B S395A
Plasmid#176005PurposeExpresses WIPI2B (S395A) fused to Halo in mammalian cellsDepositorInsertWIPI2B (S395A) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationSerine 395 to AlaninePromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJPS2955
Plasmid#135589PurposeSNR6 has UUUAUUUC at position 100–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6Δ8 has UUUAUUUC at position 100–107 deletedPromoterNativeAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p415:iCas9
Plasmid#138266PurposeExpression of mTN3-GGS6-dCas9 (iCas9) in yeast.DepositorInsertmTN3-GGS6-dCas9
UseCRISPRTagsExpressionYeastMutationCas9-D10A, H840APromoterGalLAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(NT)
Plasmid#138258PurposeExpression of a non-targeting sgRNA to be used as a control in a yeast dual reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hsg(NT:NT)
Plasmid#138260PurposeExpression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRTagsExpressionMammalianMutationPromoterNoneAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-ysg(NT)
Plasmid#138263PurposeSubcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.DepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
UL79(E48A_L49A)-3xFLAG pCDNA4
Plasmid#129759PurposeExpresses CMV UL79 (E48A_L49A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertUL79 (UL79 Human Cytomegalovirus (HHV-5))
UseTags3xFLAGExpressionMammalianMutationchanged Glutamic acid 48 to Alanine and Leucine 4…PromoterCMVAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_39J
Plasmid#91155PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:barDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3_zfh1_CP-candidate_luc+
Plasmid#86391PurposeLuciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activityDepositorInsertD. melanogaster zfh1 enhancer
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only