Addgene

We narrowed to 8,613 results for: CAG

Showing: 3101 - 3120 of 8613 results

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pB-bsd-Flag-Trl1

Plasmid

#126609

Purpose

Expresses N-terminal Flag-tagged yeast Trl1

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

tRNA ligase 1 (TRL1 Budding Yeast)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry_eDHFR_SIM#1

Plasmid

#164646

Purpose

tag SIM with mCherry and eDHFR

Depositor

Huaiying Zhang, Michael Lampson

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIM

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTAL-MajSat-FLAG-NLS-VP64

Plasmid

#69074

Purpose

Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

TALE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pT2ADW_2paRAF

Plasmid

#129654

Purpose

For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-eDHFR-SIMvada

Plasmid

#164649

Purpose

SIM mutant tagged with mCherry and eDHFR

Depositor

Huaiying Zhang, Michael Lampson

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIMvada

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet P2A Rac1-wt

Plasmid

#125135

Purpose

Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged WT Rac1.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

mNeon/mScarletP2A-Rac1

Use

Tags

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTAL-MajSat-FLAG-NLS-MCS

Plasmid

#69073

Purpose

Expresses TALE for mouse major satellite with FLAG tag under CAG promoter

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

TALE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pGST2-CeEP55

Plasmid

#21501

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

CEP55-EABR (ESCRT and ALIX Binding Region) domain (CEP55 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Q230 (CAG changed to TAA), Q218 (CAA changed to T…

Promoter

Available since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pERB257

Plasmid

#67761

Purpose

Expresses KLC1-mCherry-eDHFR

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

KLC1-mCherry-eDHFR (Klc1 Rat)

Use

Tags

eDHFR and mCherry

Expression

Mammalian

Mutation

contains Klc1 amino acids 1-175

Promoter

CAG

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Foxp2 in situ probe

Plasmid

#45620

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Foxp2 in situ probe (Foxp2 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp#1709-2142 of AF339106

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pBT241_(pCA-ATG-intron-tTA2)

Plasmid

#36875

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tTA2

Use

Tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pSLQ1653_sgSOD1

Plasmid

#63711

Purpose

Express sgSOD1 in mammalian cells of human.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

sgSOD1, CAG promoter, mCherry, puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pATM (pKD-G11)

Plasmid

#62690

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419/tdTomato

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTBL3342 PB-PEmax-puro

Plasmid

#226674

Purpose

PiggyBac cargo vector encoding PEmax and puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

PEmax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_Divergent Donor

Plasmid

#229784

Purpose

Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Convergent

Plasmid

#229790

Purpose

Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Divergent

Plasmid

#229791

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-HM4Di-YFP

Plasmid

#209193

Purpose

Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

Depositor

Larry Zweifel

Article

Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

Insert

Hm4Di

Use

AAV

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh10.1 GFP

Plasmid

#209102

Purpose

GFP expressing scramble of shRNA targeting Cdh10

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh10.1

Use

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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