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Showing: 3081 - 3100 of 3665 results

  1. pCEP4-SERTS375L-myc

    Plasmid
    #107462
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S375L with lower surface expression relative to wildtype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S375L
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107462-plasmid-map-sequence-id-205564

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  2. pCEP4-SERTA331T-myc

    Plasmid
    #107461
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A331T conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A331T
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107461-plasmid-map-sequence-id-205562

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  3. pCEP4-SERTA169D-myc

    Plasmid
    #107460
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A169D conferring gain of function phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A169D
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107460-plasmid-map-sequence-id-205558

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  4. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

    Plasmid
    #218513
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of the Plac_mrfp cassette
    Promoter
    PgyrA_ermBL-ermB'
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218513-plasmid-map-sequence-id-444102

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  5. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid
    #218514
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(1)
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218514-plasmid-map-sequence-id-444100

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  6. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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  7. pEPQD0KN0750

    Plasmid
    #185627
    Purpose
    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    lacZ
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185627-plasmid-map-sequence-id-368554

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  9. pYPQ265E2

    Plasmid
    #164719
    Purpose
    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    A3A(Y130F)-zCas9(D10A)-UGI
    Use
    CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164719-plasmid-map-sequence-id-325392

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  10. pYPQ230-RRV

    Plasmid
    #188543
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRV
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188543-plasmid-map-sequence-id-369473

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  11. pYPQ265E9

    Plasmid
    #213472
    Purpose
    Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing
    Depositor
    Yiping Qi
    Article
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadDE-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tadde-32aa linker-zspc…
    Tags
    NO
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213472-plasmid-map-sequence-id-426728

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  15. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

    Plasmid
    #52889
    Purpose
    "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression
    Depositor
    Iswar Hariharan
    Article
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Insert
    Gal4 (GAL4 Budding Yeast)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    52889-plasmid-map-sequence-id-99556

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  16. pNICKclos2.0

    Plasmid
    #73228
    Purpose
    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sgRNA to xylR
    Use
    E.coli - clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Pj23119 and Pthl
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73228-plasmid-map-sequence-id-178086

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  18. pAD-KPS12-Pman

    Plasmid
    #133832
    Purpose
    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    mKate(KPS12)
    Use
    E.coli/bacillus spp. shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pman
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133832-plasmid-map-sequence-id-261722

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  19. pEN-dCas9-CDA1-UGI

    Plasmid
    #196248
    Purpose
    Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    attL4-RecApro-dCas9-CDA1-UGI-attR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    RecA promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196248-plasmid-map-sequence-id-385887

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  20. pDEINO1

    Plasmid
    #179472
    Purpose
    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Inserts
    ori
    cat
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    179472-plasmid-map-sequence-id-375147

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Showing: 3081 - 3100 of 3665 results