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Showing: 3061 - 3080 of 4478 results

  1. PB-hEF1A-MG-U6-sgRNA

    Plasmid
    #175505
    Purpose
    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a, U6
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175505-plasmid-map-sequence-id-345098

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  7. pP2A_NLS_sfGFP

    Plasmid
    #63709
    Purpose
    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.
    Depositor
    Stanley Qi
    Article
    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.
    Insert
    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    Use
    Just a donor dna with nanog homologous arm for kn…
    Tags
    sfGFP
    Expression
    Mutation
    Promoter
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    63709-plasmid-map-sequence-id-107390

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  10. pC1.530

    Plasmid
    #205441
    Purpose
    Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.
    Depositor
    Alistair McCormick
    Article
    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:
    Insert
    RepB gRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    205441-plasmid-map-sequence-id-408759

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  12. AICSDP-144:CTCF-mEGFP

    Plasmid
    #168799
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF
    Depositor
    Allen Institute for Cell Science
    Insert
    CTCF (CTCF Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    June 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    168799-plasmid-map-sequence-id-334694

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  13. pZH521

    Plasmid
    #102674
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102674-plasmid-map-sequence-id-199585

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  15. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  19. AICSDP-079:DCP1A-mEGFP

    Plasmid
    #193922
    Purpose
    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A
    Depositor
    Allen Institute for Cell Science
    Insert
    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193922-plasmid-map-sequence-id-380539

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Showing: 3061 - 3080 of 4478 results