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Plasmid#58834PurposeExpresses GFP-Tagged Scs2 in yeastDepositorInsertScs2 (SCS2 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-C-SF-TAP-DEST-TLK1- M566T
Plasmid#218831PurposeExpresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.DepositorInsertTLK1 M566T (TLK1 Human)
UseTagsFLAG-STREPExpressionMammalianMutationM566TPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC-C-SF-TAP-DEST-TLK1- Q479E
Plasmid#218830PurposeExpresses C-terminal Strep-FLAG-tagged Q479E human TLK1 variant 5 in a retrovirus vector.DepositorInsertTLK1 Q479E (TLK1 Human)
UseTagsFLAG-STREPExpressionMammalianMutationQ479EPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-paGFP-bPAC-CAAX
Plasmid#204671PurposeMammalian expression of membrane-bound bPAC tagged with paGFP for cAMP productionDepositorArticleInsertpaGFP-bPAC-CAAX
UseLentiviralTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-rabbit-Caspase-4
Plasmid#183375PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-horse-Caspase-4
Plasmid#183380PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-wombat-Caspase-4
Plasmid#183379PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-sheep-Caspase-4
Plasmid#183376PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-lemur-Caspase-4
Plasmid#183374PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
plIVMD118
Plasmid#160938PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mCherry-FLAG tag
UseTagsMYC tagExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-active WRN
Plasmid#125788Purposeconstitutive, ectopic expression of human active WRNDepositorInsertmyc-active WRN (WRN Human)
UseTags5x mycExpressionMammalianMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN K577M
Plasmid#125790Purposeconstitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)DepositorInsertmyc-WRN K577M (WRN Human)
UseTags5x mycExpressionMammalianMutationK577M (helicase-dead)PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN E84A
Plasmid#125789Purposeconstitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)DepositorInsertmyc-WRN E84A (WRN Human)
UseTags5x mycExpressionMammalianMutationE84A (exonuclease-dead)PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-FTO promoter_WT
Plasmid#108922PurposeThe core promoter region of FTO was inserted into pGL3 basic vectorDepositorInsertFTO (FTO Human)
UseTagsNAExpressionMammalianMutationNAPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-FTO promoter_Mut
Plasmid#108923PurposeThe core promoter region of FTO with mutant CEBPA binding sites was inserted into pGL3 basic vectorDepositorInsertFTO (FTO Human)
UseTagsNAExpressionMammalianMutationNAPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
ML8
Bacterial Strain#61909PurposeC43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. argH gene deleted.DepositorBacterial ResistanceKanamycinAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
ML3
Bacterial Strain#61907PurposeC43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG gene deleted.DepositorBacterial ResistanceKanamycinAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Jun lab tunable CRISPRi strain
Bacterial Strain#86400PurposeStrain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-termDepositorBacterial ResistanceSpectinomycin and hygromycinAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
ML2
Bacterial Strain#61906PurposeC43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.DepositorBacterial ResistanceKanamycinAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
E. coli MEV20
Bacterial Strain#197113PurposeE. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette clusterDepositorBacterial ResistanceNoneAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only