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pC0.425
Plasmid#203944PurposeUp Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.DepositorInsertCas12a UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.426
Plasmid#203945PurposeDown Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.DepositorInsertCas12a DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.391
Plasmid#203948PurposeLevel 0 part. Cas12a coding sequence from Francisella novicida in CDS1 positionDepositorInsertFnCas12a
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.420
Plasmid#203934PurposeUp Flank. Neutral site. Up flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901.DepositorInsertaquI UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.385
Plasmid#203936PurposeUp Flank. Neutral site. Up flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901.DepositorInsertdesB UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.386
Plasmid#203938PurposeUp Flank. Neutral site. Up flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901.DepositorInsertglgA1 UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.387
Plasmid#203939PurposeDown Flank. Neutral site. Down flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901.DepositorInsertglgA1 DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.388
Plasmid#203940PurposeUp Flank. Neutral site. Up flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.DepositorInsertmrr UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.389
Plasmid#203941PurposeDown Flank. Neutral site. Down flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.DepositorInsertmrr DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.437
Plasmid#203942PurposeUp Flank. Neutral site. Up flanking sequence of intergenic region (NS1) between FEK30_11550 and FEK30_11555 in Synechococcus sp. PCC 11901.DepositorInsertNS1 UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
aCE89
Plasmid#204494PurposeTREtight promoted WBeta and PhlF promoted PhiC31 without PEST tagsDepositorInsertsφC31 integrase
WBeta recombinase
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterPhIF and TREtightAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
aCE90
Plasmid#204495PurposeTREtight promoted WBeta and PhlF promoted PhiC31 with PEST tagsDepositorInsertsphiC31 recombinase
WBeta recombinase
UseSynthetic BiologyTagsPESTExpressionBacterial and MammalianMutationPromoterPhIF and TREtightAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
aCE106
Plasmid#204496PurposeSmall molecule helper circuitDepositorInsertsrtTA3
PhIF-ABI
VPR-Pyl1
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterhEF1aAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMP065
Plasmid#207929PurposePremade plasma membrane marker, pUBQ10:PiP1;4:mVenus:tHSP18.2M, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTB3-FLAG tag
Plasmid#210204PurposeType 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.DepositorInsertFLAG-tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTB3-AviD tag
Plasmid#210203PurposeType 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.DepositorInsertAviD-tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTB3-Myc tag
Plasmid#210205PurposeType 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.DepositorInsertMyc-tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMP086
Plasmid#207930PurposePremade plasma membrane marker, pUBQ10:PiP1;4:mVenus:tHSP18.2M:FastRed, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKD013
Plasmid#207931PurposePremade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only