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Plasmid#67154PurposepEGFP containing N-terminally EGFP Tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1346 U6-B2M sgRNA Gag-pol v2
Plasmid#201917PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorInsertsGag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
UseTagsExpressionMammalianMutationPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_H1_Gal4VP64
Plasmid#169918PurposeExpression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.DepositorInsertantiCD19-Notch1-GL4VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-5102HRasCT(Picchu)
Plasmid#209917PurposeA FRET biosensor for tyrosine kinase activityDepositorInsert5102HRasCT
UseTagsYPet and nTurquoise-GLExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
TurboID-CRaf
Plasmid#226168PurposeLentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.DepositorInsertMyc-TurboID-CRaf (RAF1 Human)
UseLentiviralTagsEGFP-IRES-BlastR and Myc-TurboIDExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro
Plasmid#211687PurposeU6-sgRNA-EFS-Cas9-P2A-PuroDepositorInsertSpyCas9 and guide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1365 LV EF1a-CD28 IRES-EGFP
Plasmid#201921Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD28DepositorInsertCD28 (CD28 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_ORI vector
Plasmid#99296PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-Kif5b-Myc
Plasmid#186614PurposeThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tagDepositorInsertKinesin Heavy Chain isoform 5b (KIF5B Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR
Plasmid#64927PurposeFRET-based biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR (EIF4EBP1 Synthetic, Human)
UseTagsCerulean and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
DT960
Plasmid#80412PurposeExpresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-H
Plasmid#182281PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.DepositorInsertcAMPFIRE-H (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-GFP
Plasmid#40125DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
DT480
Plasmid#80413PurposeExpresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK3_038
Plasmid#123752PurposeEncodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK systemDepositorInsertR-FlincA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
DT0
Plasmid#80418PurposeExpresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD3
Plasmid#24167PurposeMammalian expression of human JMJD3 with HA tagDepositorInsertJMJD3 (KDM6B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only