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Plasmid#225696PurposeLentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.DepositorInsertosTIR1
UseLentiviral and Synthetic BiologyTags9X MycExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8270 LentiCRISPR v2 hygro sgSIGIRR-3
Plasmid#193979PurposeExpression of spCas9 and sgRNA targeting SIGIRRDepositorInsertspCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUG-IRES-mCherry
Plasmid#222109PurposeStart codon reporter (WT AUG)DepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKanB
Plasmid#223523PurposeUsed for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAOX1Available sinceSept. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMBA329
Plasmid#214749PurposeHigh copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA328
Plasmid#214748PurposeMedium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA327
Plasmid#214747PurposeLow copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nc-NylC_pET28a
Plasmid#215412PurposeExpression construct for Nc-NylC gene, codon optimized for expression in E. coliDepositorInsertNc-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA325
Plasmid#214742PurposeMedium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA324
Plasmid#214741PurposeLow copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA326
Plasmid#214743PurposeHigh copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSV014
Plasmid#214740PurposeHigh copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSV013
Plasmid#214739PurposeMedium copy thyA-infA plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSV015
Plasmid#214738PurposeLow copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY380
Plasmid#182962PurposeAmp-resistant, low copy (p15A ori), E. coli 5'ptsI transcriptionally fused with GFP.DepositorInsertptsI
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY390
Plasmid#182963PurposeAmp-resistant, low copy (p15A ori), E. coli ptsI transcriptionally fused with GFP.DepositorInsertptsI
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP765_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IF_lacZ
Plasmid#192409PurposeTo act as a 1-input state of the A NIMPLY B gate.DepositorInsertB3RT-Act2-B3RT::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-TP-mCherry-UnaG
Plasmid#203483PurposeFor Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertPlastid transit peptide fused mCherry-UnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
UseTagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-PAH1-3HA
Plasmid#184761PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorInsertPAH1 (PAH1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterPAH1Available sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only