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Plasmid#157982PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Tetra-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(K72A)-IRES-puro
Plasmid#128661Purposemammalian expression of Flag-Polß(K72A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationK72APromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(K244A)-IRES-puro
Plasmid#128682Purposemammalian expression of Flag-Polß(K244A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationK244APromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(L301R)-IRES-puro
Plasmid#128654Purposemammalian expression of Flag-Polß(L301R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301RPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(K206A)-IRES-puro
Plasmid#128678Purposemammalian expression of Flag-Polß(K206A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationK206APromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM-K206A)-IRES-puro
Plasmid#128679Purposemammalian expression of Flag-Polß(TM-K206A: L301R/V303R/V306R-K206A) protein with puromycin slectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306R/K206APromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM-KTM)-IRES-puro
Plasmid#128676Purposemammalian expression of Flag-Polß(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306R/-K41R/K61R/K81RPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(V306R)-IRES-puro
Plasmid#128655Purposemammalian expression of Flag-Polß(V306R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationV306RPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(KTM)-IRES-puro
Plasmid#128672Purposemammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationK41R/K61R/K81RPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM-K244A)-IRES-puro
Plasmid#128680Purposemammalian expression of Flag-Polß(TM-K244A: L301R/V303R/V306R-K244A) portein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306R/K244APromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNA
Plasmid#117407PurposeNmeCas9 sgRNA targeting TLR2.0DepositorInsertNmeCas9 sgRNA targeting TLR 2.0
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-U6-CjeCas9-TLR-MCV1-sgRNA
Plasmid#117408PurposeCjeCas9 sgRNA targeting TLR2.0DepositorInsertCjeCas9 sgRNA targeting TLR 2.0
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNA
Plasmid#117409PurposeSauCas9 sgRNA targeting TLR2.0DepositorInsertSauCas9 sgRNA targeting TLR 2.0
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA
Plasmid#117410PurposeLbCas12a-gRNA targeting TLR2.0DepositorInsertLbCas12a gRNA targeting TLR 2.0
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3
Plasmid#128764PurposeExpresses DIS3L2 gRNA for CRISPRiDepositorInsertDIS3L2 sgRNA #3 (DIS3L2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2
Plasmid#128763PurposeExpresses DIS3L2 gRNA for CRISPRiDepositorInsertDIS3L2 sgRNA #2 (DIS3L2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1
Plasmid#128762PurposeExpresses DIS3L2 gRNA for CRISPRiDepositorInsertDIS3L2 sgRNA #1 (DIS3L2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2
Plasmid#128760PurposeExpresses PARN gRNA for CRISPRiDepositorInsertPARN sgRNA #2 (PARN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3
Plasmid#128758PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #3 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1
Plasmid#128756PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #1 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only