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Plasmid#96943PurposeThe plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).DepositorInsertBE3∆UGI
UseTagsHis6 tagExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
STK3_HUMAN_D0
Plasmid#79689PurposeThis plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK3 (STK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG
Plasmid#74261PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.Y591X truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ABE-NT-sgRNA
Plasmid#112734PurposeAAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNADepositorInsertABE-NT-sgRNA
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PGAM1_sgRNA2
Plasmid#201615PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPGAM1 (PGAM1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (NZF1, aa 1-33)
Plasmid#65437PurposeExpresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coliDepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GSTExpressionBacterialMutationonly codes for amino acids 1-33 of TRABID, which …PromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-ITFG2
Plasmid#87045Purposemammalian expression of ITFG2DepositorInsertITFG2 (ITFG2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-Ataxin3Q22
Plasmid#22117DepositorInsertAtaxin 3 (ATXN3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-hCD24-T2A-GFP
Plasmid#205456Purposelentiviral plasmid for expression of human CD24DepositorInserthCD24 (CD24 Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PAK7_HUMAN_D0
Plasmid#79706PurposeThis plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK7 (PAK5 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG
Plasmid#132685PurposeAAV plasmid encoding mouse CNDP2 with C-terminal flag tagDepositorInsertCarnosine dipeptidase 2 (Cndp2 Mouse)
UseAAVTagsFlagExpressionMutationPromoterChicken b-actinAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
PLEX_313 GFP
Plasmid#184494PurposeLentivirus expression for GFPDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
VGFR1_HUMAN_D0
Plasmid#79743PurposeThis plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVGFR1
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf117
Plasmid#12805PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf117
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT-TO-GFP-PALB2 KR
Plasmid#71107Purposemammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)DepositorInsertPALB2 (PALB2 Human)
UseTagsGFPExpressionMammalianMutationfirst 8 Lysine residues mutated to Arginine (K14-…PromoterCMV/TOAvailable sinceDec. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
TRE-UBC-Crry-T2A-GFP
Plasmid#205452Purposelentiviral plasmid for expression of mouse CrryDepositorInsertCrry (Cr1l Mouse)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-MAVS-5'UTR
Plasmid#184639PurposeFluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTRDepositorInsertmScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK5-MYC-ITFG2
Plasmid#87046Purposemammalian expression of ITFG2DepositorInsertITFG2 (ITFG2 Human)
UseTagsMYCExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
plc242-GFP-ITFG2
Plasmid#87049Purposemammalian expression of ITFG2DepositorInsertITFG2 (ITFG2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only