-
Plasmid#125460PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7732130-guide4
Plasmid#125447PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs1401419-guide2
Plasmid#125425PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide4
Plasmid#125396PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs11257655-guide3
Plasmid#118206PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide3
Plasmid#118200PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs7903146-guide3
Plasmid#118204PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3
Plasmid#118202PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1
Plasmid#118169PurposeCRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118163PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide3
Plasmid#125398PurposeCRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide1
Plasmid#125393PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide2
Plasmid#118178PurposeCRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide4
Plasmid#118180PurposeCRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide4
Plasmid#118172PurposeCRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide1
Plasmid#118177PurposeCRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
eScaf
Bacterial Strain#220921PurposecssDNA production strainDepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
S4246
Bacterial Strain#156372PurposeErythromycin-resistant S2060 derivativeDepositorBacterial ResistanceStreptomycin, tetracycline, erythromycinAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Syn61
Bacterial Strain#174513PurposeRecoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1DepositorBacterial ResistanceStreptomycinAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
JEC027
Bacterial Strain#180310PurposeE. coli MG1655 strain with knockout of endogenous Type I-E CRISPR Cas systemDepositorBacterial ResistanceNoneAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only