Addgene

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pSIN-Arkadia-gRNA3

Plasmid

#180364

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-MYH10_sgRNA

Plasmid

#183887

Purpose

pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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cASPG1-mRFP

Plasmid

#181962

Purpose

ASPG1 protein tagged with mRFP, under control of TBA2 promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

ASPG1 (ASPG1 Mustard Weed)

Use

Tags

mRFP

Expression

Plant

Mutation

Promoter

TBA2 promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-440-V5His6_D

Plasmid

#146123

Purpose

Insect Expression of DmEDC3_1-440

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-440 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pG418R-MCS-LexA-VP16-MiniWhite

Plasmid

#165894

Purpose

G418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

pCMV and T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMRBad-C-DiB-RM

Plasmid

#168478

Purpose

C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

C-fragment of DiB-RM

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-synPCB2.2

Plasmid

#139479

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG

Use

Tags

Expression

Mammalian

Mutation

V554L

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_019

Plasmid

#123800

Purpose

Encodes dsaCAS9 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SadCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3_028

Plasmid

#124054

Purpose

Encodes c120 sg RNA guide cleaved by ribozyme

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RIbozyme (C120 sg RNA)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8b_006

Plasmid

#123894

Purpose

Encodes the LoxP recognition site as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LoxP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_006

Plasmid

#123874

Purpose

Encodes the FRT cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

FRT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK6_006

Plasmid

#123860

Purpose

Encodes a spacer cassette as a type 6 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_008

Plasmid

#123822

Purpose

Encodes Golgi Targeting Sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_012

Plasmid

#123825

Purpose

Encodes mScarlet as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_015

Plasmid

#123828

Purpose

Encodes msfGFP as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

msfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_023

Plasmid

#123835

Purpose

Encodes Gibberellin Insensitive as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GAI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3b_018

Plasmid

#123799

Purpose

Encodes dspCAS9 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SpdCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3b_028

Plasmid

#123806

Purpose

Encodes Venus as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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