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Showing: 3001 - 3020 of 150071 results

  3. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

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  8. pCS2+ H2B-mCerulean

    Plasmid
    #99268
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mCerulean in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription
    Depositor
    Philipp Keller
    Insert
    mCerulean-3XFLAG
    Use
    Tags
    H2B
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    99268-plasmid-map-sequence-id-192965

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  9. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61408-plasmid-map-sequence-id-333613

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  10. pGGF012

    Plasmid
    #48849
    Purpose
    Provides a sulfadiazine resistance cassette as GreenGate module.
    Depositor
    Jan Lohmann
    Article
    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:
    Insert
    pMAS::SulfadiazineR::t35S
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    internal BsaI recognition sites in SulfR removed …
    Promoter
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    48849-plasmid-map-sequence-id-71266

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  16. pRL443

    Plasmid
    #70261
    Purpose
    Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70261-plasmid-map-sequence-id-348706

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Showing: 3001 - 3020 of 150071 results