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LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

Plasmid

#192413

Purpose

To act as a 1-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-CDX2-MUT

Plasmid

#16554

Depositor

Bert Vogelstein

Article

da Costa et al Oncogene. 1999 Sep 2. 18(35):5010-4.

Insert

CDX2 (CDX2 Human)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

RKO mutation

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only
pYC121

Plasmid

#63917

Purpose

Recipient overexpressing vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

MYC

Expression

Yeast

Mutation

Promoter

TEF1 (Saccharomyces cerevisiae)

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-L1PA8WTmneo

Plasmid

#69609

Purpose

Expresses a neomycin tagged wildtype L1PA8

Depositor

Astrid Roy-Engel

Article

Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

Insert

L1PA8

Use

Tags

mneo retrotransposition cassette

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

Plasmid

#21800

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Use

Tags

GST and TEV

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I-delta N90

Plasmid

#22165

Depositor

Ananda Roy

Article

Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

Insert

TFII-I (GTF2I Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

The first 90 aa of TFII-I are deleted

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B R108E

Plasmid

#176004

Purpose

Expresses WIPI2B (R108E) fused to Halo in mammalian cells

Depositor

Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (R108E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Arginine 108 to Glutamate

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-CDS1-3HA

Plasmid

#198596

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-DGK1-3HA

Plasmid

#198595

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-PAH1-3HA

Plasmid

#198597

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBS B2rescue A70D

Plasmid

#78658

Purpose

Generates B2 inserts suitable for rescue

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

Insert

B2rescue cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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ORF31-2xStrep pCDNA4

Plasmid

#129744

Purpose

Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF31 (ORF31 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.TM0851

Plasmid

#11530

Depositor

Sung-Hou Kim

Article

Liu et al J Mol Biol. 2005 Jul 29. 350(5):987-96.

Insert

heat-inducible transcriptional repressor (TM0851 T. maritima)

Use

Tags

HisTag, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pYC119

Plasmid

#63916

Purpose

Recipient overexpressing vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

TEF1 (Saccharomyces cerevisiae)

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192396

Purpose

To act as a 2-input state of the AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192391

Purpose

To act as a 2-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST(frt)(ATG_V5-APEX2_noSTOP)

Plasmid

#168207

Purpose

Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Type

Empty backbone

Use

Unspecified; Pdest gateway

Tags

V5 APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ORF30-2xStrep pCDNA4

Plasmid

#129743

Purpose

Expresses KSHV ORF30 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF30 (ORF30 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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2xStrep-ORF34 pCDNA4

Plasmid

#120376

Purpose

Expresses KSHV ORF34 with Nterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF34 (ORF34 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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