Addgene

We narrowed to 6,285 results for: sms

Showing: 2981 - 3000 of 6285 results

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pDEST-FRT/T0-GFP-BRCA1

Plasmid

#71116

Purpose

mammalian expression of GFP-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-mScarlet-Control-5'UTR

Plasmid

#184637

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 HA Arf1 DN T31N

Plasmid

#10833

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 T31N (ARF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T31N. Defective in GTP binding. Dominant negati…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-cIAP2/pRK5

Plasmid

#27973

Depositor

Xiaolu Yang

Article

Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

Insert

CIAP2 (BIRC3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pMP71-mIL15-IL12rb1-sushi-il12rb2

Plasmid

#174606

Purpose

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRCH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-BRCA1

Plasmid

#99394

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

Depositor

Michael Huen, Junjie Chen

Article

Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

BRCA1 (BRCA1 Human)

Use

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Diacylglycerol Kinase Zeta

Plasmid

#85454

Purpose

expression of Diacylglycerol Kinase Zeta

Depositor

Stephen Gee

Article

Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

Insert

Diacylglycerol Kinase Zeta (DGKZ Human)

Use

Tags

3x HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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BAP1

Plasmid

#81024

Purpose

Retroviral vector containing human BAP1 and Thy1.1 selection marker

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5’LTR

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf170

Plasmid

#12858

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jon Yewdell, Jack Bennink

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf170

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax-NRTH

Plasmid

#136922

Purpose

Expresses ABEmax-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRTH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-Flag-BRCA1

Plasmid

#71117

Purpose

mammalian expression of Flag-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Nov. 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

Plasmid

#89508

Purpose

Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

Insert

rAPOBEC1-nCas9 (D10A)-UGI-NLS

Use

Tags

UGI and rAPOBEC-XTEN

Expression

Bacterial

Mutation

D10A mutation of Cas9

Promoter

T7

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin2

Plasmid

#72595

Purpose

overexpression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN2 (SESN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
NEK7_HUMAN_D0

Plasmid

#79735

Purpose

This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK7 (NEK7 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
PIK3CA-WT (pFastBac)

Plasmid

#16644

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-BE4max-NRRH

Plasmid

#136918

Purpose

Expresses BE4max-NRRH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRRH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRCH

Plasmid

#136920

Purpose

Expresses BE4max-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRCH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRTH

Plasmid

#136919

Purpose

Expresses BE4max-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRTH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge